Bioengineered adipose-derived stem cells for targeted enzyme-prodrug therapy of ovarian cancer intraperitoneal metastasis

JOURNAL OF CONTROLLED RELEASE

卷:311

Article

Malekshah, Obeid M.¹  Sarkar, Siddik¹  Nomani, Alireza¹  Patel, Niket¹  Javidian, Parisa²  Goedken, Michael³  Polunas, Marianne³  Louro, Pedro³  Hatefi, Arash^1,4 

[1] Rutgers State Univ, Dept Pharmaceut, Room 222,160 Frelinghuysen Rd, Piscataway, NJ 08854 USA

[2] Rutgers Robert Wood Johnson Med Sch, Dept Pathol & Lab Med, New Brunswick, NJ 08903 USA

[3] Rutgers State Univ, Rutgers Res Pathol Serv, Piscataway, NJ 08854 USA

[4] Rutgers Canc Inst New Jersey, Canc Pharmacol Program, New Brunswick, NJ 08903 USA

关键词: Adipose derived mesenchymal stem cells;    Suicide gene therapy;    Ovarian cancer;    Targeted therapy;    Enzyme prodrug systems;   

DOI  :  10.1016/j.jconrel.2019.09.006

来源: Elsevier

【 摘 要 】

The objective of this study was to develop a stem cell-based system for targeted suicide gene therapy of recurrent, metastatic, and unresectable ovarian cancer. Malignant cells were obtained from the ascites of a patient with advanced recurrent epithelial ovarian cancer (named OVASC-1). Cancer cells were characterized to determine the percentages of drug-resistant ALDH + cells, MDR-1/ABCG2 overexpressing cells, and cancer stem-like cells. The sensitivity and resistance of the OVASC-1 cells and spheroids to the metabolites of three different enzyme/prodrug systems were assessed, and the most effective one was selected. Adipose-derived stem cells (ASCs) were genetically engineered to express recombinant secretory human carboxylesterase-2 and nanoluciferase genes for simultaneous disease therapy and quantitative imaging. Bioluminescent imaging, magnetic resonance imaging and immuno/histochemistry results show that the engineered ASCs actively targeted and localized at both tumor stroma and necrotic regions. This created the unique opportunity to deliver drugs to not only tumor supporting cells in the stroma, but also to cancer stem-like cells in necrotic/hypoxic regions. The statistical analysis of intraperitoneal OVASC-1 tumor burden and survival rates in mice shows that the administration of the bioengineered ASCs in combination with irinotecan prodrug in the designed sequence and timeline eradicated all intraperitoneal tumors and provided survival benefits. In contrast, treatment of the drug-resistant OVASC-1 tumors with cisplatin/paclitaxel (standard-of-care) did not have any statistically significant benefit. The histopathology and hematology results do not show any toxicity to major peritoneal organs. Our toxicity data in combination with efficacy outcomes delineate a nonsurgical and targeted stem cell-based approach to overcoming drug resistance in recurrent metastatic ovarian cancer.

【 授权许可】

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