Co-delivery of GPI-anchored CCL28 and influenza HA in chimeric virus-like particles induces cross-protective immunity against H3N2 viruses

JOURNAL OF CONTROLLED RELEASE

卷:233

Article

Mohan, Teena^1,2  Kim, Jongrok¹  Berman, Zachary^1,2  Wang, Shelly¹  Compans, Richard W.¹  Wang, Bao-Zhong^1,2 

[1] Emory Univ, Dept Microbiol & Immunol, Sch Med, 1518 Clifton Rd, Atlanta, GA 30322 USA

[2] Georgia State Univ, Inst Biomed Sci, Ctr Inflammat Immun & Infect, 100 Piedmont Ave SE, Atlanta, GA 30303 USA

关键词: Antibody avidity;    Hemagglutination;    Influenza;    Mucosal adjuvant;    Neutralization;    Virus-like particles (VLPs);   

DOI  :  10.1016/j.jconrel.2016.05.021

来源: Elsevier

【 摘 要 】

Influenza infection typically initiates at respiratory mucosal surfaces. Induction of immune responses at the sites where pathogens initiate replication is crucial for the prevention of infection. We studied the adjuvanticity of GPI-anchored CCL28 co-incorporated with influenza HA-antigens in chimeric virus-like particles (cVLPs), in boosting strong protective immune responses through an intranasal (i.n.) route in mice. We compared the immune responses to that from influenza VLPs without CCL28, or physically mixed with soluble CCL28 at systemic and various mucosal compartments. The cVLPs containing GPI-CCL28 showed in-vitro chemotactic activity towards spleen and lung cells expressing CCR3/CCR10 chemokine receptors. The cVLPs induced antigen specific endpoint titers and avidity indices of IgG in sera and IgA in tracheal, lung, and intestinal secretions, significantly higher (46 fold) than other formulations. Significantly higher (3-5 fold) hemagglutination inhibition titers and high serum neutralization against H3N2 viruses were also detected with CCL28-containing VLPs compared to other groups. The CCL28-containing VLPs showed complete and 80% protection, when vaccinated animals were challenged with A/Aichi/2/1968/H3N2 (homologous) and A/Philippines/2/1982/H3N2 (heterologous) viruses, respectively. Thus, GPI-anchored CCL28 in influenza VLPs act as a strong immunostimulator at both systemic and mucosal sites, boosting significant cross-protection in animals against heterologous viruses across a large distance. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

Free