Thirdhand smoke component can exacerbate a mouse asthma model through mast cells

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:142

Article

Yu, Mang^1,2  Mukai, Kaori^1,2  Tsai, Mindy^1,2  Galli, Stephen J.^1,2,3 

[1] Stanford Univ, Sch Med, Dept Pathol, 269 Campus Dr,Rm 3255, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Sean N Parker Ctr Allergy & Asthma Res, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

关键词: Thirdhand smoke;    4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone;    mast cells;    alpha 7 nicotinic acetylcholine receptor;    cockroach allergen;   

DOI  :  10.1016/j.jaci.2018.04.001

来源: Elsevier

【 摘 要 】

Background: Thirdhand smoke (THS) represents the accumulation of secondhand smoke on indoor surfaces and in dust, which, over time, can become more toxic than secondhand smoke. Although it is well known that children of smokers are at increased risk for asthma or asthma exacerbation if the disease is already present, how exposure to THS can influence the development or exacerbation of asthma remains unknown. Objective: We investigated whether epicutaneous exposure to an important component of THS, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), can influence asthma pathology in a mouse model elicited by means of repeated intranasal challenge with cockroach antigen (CRA). Methods: Wild-type mice, alpha 7 nicotinic acetylcholine receptor (nAChR)-or mast cell (MC)-deficient mice, and mice with MCs that lacked alpha 7 nAChRs or were the host's sole source of alpha 7 nAChRs were subjected to epicutaneous NNK exposure, intranasal CRA challenge, or both, and the severity of features of asthma pathology, including airway hyperreactivity, airway inflammation, and airway remodeling, was assessed. Results: We found that alpha 7 nAChRs were required to observe adverse effects of epicutaneous NNK exposure on multiple features of CRA-induced asthma pathology. Moreover, MC expression of alpha 7 nAChRs contributed significantly to the ability of epicutaneous NNK exposure to exacerbate airway hyperreactivity to methacholine, airway inflammation, and airway remodeling in this model. Conclusion: Our results show that skin exposure to NNK, a component of THS, can exacerbate multiple features of a CRA-induced model of asthma in mice and define MCs as key contributors to these adverse effects of NNK.

【 授权许可】

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