Characteristics of lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years of age

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:126

Article

Ananworanich, Jintanat^1,2,3  Apornpong, Tanakorn¹  Kosalaraksa, Pope⁴  Jaimulwong, Tanyathip¹  Hansudewechakul, Rawiwan⁵  Pancharoen, Chitsanu³  Bunupuradah, Torsak¹  Chandara, Mom⁶  Puthanakit, Thanyawee^1,3  Ngampiyasakul, Chaiwat⁷  Wongsawat, Jurai⁸  Kanjanavanit, Suparat⁹  Luesomboon, Wicharn¹⁰  Klangsinsirikul, Phennapha¹¹  Ngo-Giang-Huong, Nicole¹²  Kerr, Stephen J.^1,13  Ubolyam, Sasiwimol¹  Mengthaisong, Tawan¹  Gelman, Rebecca S.^14,15  Pattanapanyasat, Kovit¹⁶  Saphonn, Vonthanak^6,17  Ruxrungtham, Kiat^1,3  Shearer, William T.¹⁸ 

[1] HIV Netherlands Australia Thailand Res Collaborat, Thai Red Cross AIDS Res Ctr, Bangkok, Thailand

[2] SE Asia Res Collaborat Hawaii, Bangkok, Thailand

[3] Chulalongkorn Univ, Fac Med, Bangkok 10330, Thailand

[4] Khon Kaen Univ, Khon Kaen, Thailand

[5] Chiangrai Prachanukroh Hosp, Chiang Rai, Thailand

[6] Natl Inst Publ Hlth, Phnom Penh, Cambodia

[7] Prapokklao Hosp, Chanthaburi, Thailand

[8] Bamrasnaradura Infect Dis Inst, Nonthaburi, Thailand

[9] Nakornping Hosp, Chiang Mai, Thailand

[10] Queen Savang Vadhana Mem Hosp, Chon Buri, Thailand

[11] Chiang Mai Univ, Fac Associated Med Sci, Chiang Mai, Thailand

[12] IRD U174 Program HIV Prevent & Treatment, Chiang Mai, Thailand

[13] Natl Ctr HIV Epidemiol & Clin Res, Sydney, NSW, Australia

[14] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA

[15] Dana Farber Canc Inst, Boston, MA 02115 USA

[16] Mahidol Univ, Fac Med, Siriraj Hosp, Bangkok 10700, Thailand

[17] Natl Ctr HIV AIDS Dermatol & STDs, Phnom Penh, Cambodia

[18] Baylor Coll Med, Houston, TX 77030 USA

关键词: HIV;    children;    lymphocyte;    monocyte;    phenotyping;    long-term nonprogressors;    antiretroviral therapy;    Asia;    disease progression;    pediatric AIDS;   

DOI  :  10.1016/j.jaci.2010.09.038

来源: Elsevier

【 摘 要 】

Background: There are limited data on the immune profiles of HIV-positive children compared with healthy controls, and no such data for Asian children. Objectives: To immunophenotype HIV-positive Asian children, including long-term nonprogressors (LTNPs), compared with age-matched healthy controls. Methods: We used flow cytometry to analyze 13 lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4(+) T cells and no AIDS-related illnesses and 142 healthy children (controls). Data were compared among age categories. Profiles from LTNPs (n = 50), defined as children >= 8 years old with CD4(+) T-cell counts >= 350 cells/mm(3), were compared with data from age-matched non-LTNPs (n = 17) and controls (n = 53). Results: Compared with controls, HIV-positive children had lower values (cell count per mm(3) and percent distribution) for T-H cells and higher values for cytotoxic T cells, with reductions in populations of naive T-H and cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated T-H and cytotoxic T cells. Compared with non-LTNPs, LTNPs had higher values of T-H and cytotoxic T cells, naive and memory T-cell subsets, and B and NK cells. Surprisingly, counts of activated T-H and cytotoxic T cells were also higher among LTNPs. LNTPs were more frequently male. Conclusion: Untreated, HIV-infected Asian children have immune profiles that differ from those of controls, characterized by low values for T-H cells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated T-H, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal studies. (J Allergy Clin Immunol 2010;126:1294-301.)

【 授权许可】

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