Genetic variation in chitinase 3-like 1 (CHI3L1) contributes to asthma severity and airway expression of YKL-40

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:136

Article

Gomez, Jose L.^1,2  Crisafi, Gina M.³  Holm, Carole T.^1,2  Meyers, Deborah A.⁴  Hawkins, Gregory A.⁴  Bleecker, Eugene R.⁴  Jarjour, Nizar³  Cohn, Lauren^1,2  Chupp, Geoffrey L.^1,2 

[1] Yale Univ, Sch Med, Sect Pulm Crit Care & Sleep Med, New Haven, CT 06520 USA

[2] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06520 USA

[3] Univ Wisconsin, Sch Med, Sect Pulm & Crit Care Med, Madison, WI USA

[4] Wake Forest Sch Med, Ctr Human Genom & Personalized Med, Winston Salem, NC USA

关键词: Asthma;    asthma severity;    severe asthma;    genetic association studies;    genetic variation;    CHI3L1 protein;    human;    YKL-40 protein;    human;    airway remodeling;   

DOI  :  10.1016/j.jaci.2014.11.027

来源: Elsevier

【 摘 要 】

Background: Single nucleotide polymorphisms (SNPs) in the chitinase 3-like 1 (CHI3L1) promoter, the gene encoding YKL-40, are associated with circulating YKL-40 levels and asthma prevalence. However, the effects of gene polymorphisms on asthma severity and airway expression of YKL-40 have not been examined. Objective: We sought to determine the effect of genetic variation in CHI3L1 on asthma severity and YKL-40 expression in subjects from the Yale Center for Asthma and Airways Disease and the Severe Asthma Research Program. Methods: SNPs spanning the CHI3L1 gene were genotyped in 259 Yale Center for Asthma and Airways Disease and 919 Severe Asthma Research Program subjects. Association and haplotype analyses were conducted to identify effects on airflow obstruction, YKL-40 levels, and asthma severity. Results: Fifteen SNPs in CHI3L1 were associated with FEV1, serum YKL-40 levels, or both. rs12141494 (intron 6) was the only SNP in subjects of European ancestry in both cohorts that was associated with serum YKL-40 levels and postbronchodilator FEV1. Conditional analysis demonstrated that the effect on lung function was independent of the promoter SNP rs4950928, and haplotype analysis demonstrated that G alleles at rs12141494 and rs4950928 are associated with lower YKL-40 expression and higher FEV1 percent predicted values. In asthmatic subjects the risk allele A at rs12141494 was associated with severe asthma and higher YKL-40 expression in the airway (P <= .05). Conclusion: In contrast to the promoter SNP rs4950928, the intronic SNP rs12141494 in CHI3L1 is associated with asthma severity, lung function, and YKL-40 expression in the blood and airway. These data suggest that SNP rs12141494 modulates YKL-40 expression in the airway and contributes to airway remodeling and asthma severity.

【 授权许可】

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