DISTRIBUTION OF AIRBORNE MOUSE ALLERGEN IN A MAJOR MOUSE BREEDING FACILITY

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:94

Article

OHMAN, JL ; HAGBERG, K ; MACDONALD, MR ; JONES, RR ; PAIGEN, BJ ; KACERGIS, JB

关键词: ELISA;    MUS M I;    VENTILATION;    PARTICULATE DISTRIBUTION;    AIR SAMPLING;   

DOI  :  10.1016/0091-6749(94)90147-3

来源: Elsevier

【 摘 要 】

Background: Occupational allergy to mice is a major cause of disability among workers in mouse breeding and research facilities. Efforts to prevent and treat allergy require a detailed knowledge of exposure levels to allergen. Objective: This study was designed to quantitate the level of major mouse allergen (Mus m I) in central room air and immediate breathing zones under a variety of working conditions. Methods: An Andersen sampler (Groseby Andersen, Spirotech Div., Atlanta, Ga.) was used to collect allergen in each room. A Gillian Personal sampler (Gillian Instrument Corp., West Caldwell, N.J.) collected particles in the worker breathing zone. ELISA was used to quantitate the concentration of Mus m I collected on the two collection devices. Results: Total Mus m I recovered from Andersen samplers ranged from 0.2 to 1.5 ng/m(3) irt rooms without mice and 0.5 to 15.1 ng/m(3) in rooms with mice. Allergen recovered from the zone of worker activity ranged from 1.2 to 2.7 ng/m(3) in rooms without mice and from 16.6 to 563.0 ng/m(3) in rooms with mice. Direct mouse contact was associated with the highest levesl of exposure to Mus m I. Analysis revealed the bulk of allergen to be in mid-particle size ranges (3.3 to 10 mu m) for mouse-containing rooms and in small particle size range (0.43 to 3.3 mu m) for non-mouse-containing rooms, suggesting that small particles were carried along corridors from rooms with mice into non-mouse-containing rooms. Ventilation characteristics of rooms and mouse population density were evaluated with a ''mouse loading'' index (number of mice per cubic meter of ventilated air per hour). Mouse loading correlated strongly with small particles (<3.3 mu m) in ambient air. Conclusions: Mus m I is widely distributed within mouse breeding facilities. Direct worker contact with mice seems to be the major factor in high level exposure.

【 授权许可】

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