Bet v 1142-156 is the dominant T-cell epitope of the major birch pollen allergen and important for cross-reactivity with Bet v 1-related food allergens

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:116

Article

Jahn-Schmid, B ; Radakovics, A ; Lüttkopf, D ; Scheurer, S ; Vieths, S ; Ebner, C ; Bohle, B

关键词: birch pollen allergy;    food allergy;    oral allergy syndrome;    PR-10;    Bet v 1;    T-cell cross-reactiviry;   

DOI  :  10.1016/j.jaci.2005.04.019

来源: Elsevier

【 摘 要 】

Background: Individuals with birch pollen allergy frequently experience hypersensitivity reactions to certain foods, primarily because of IgE antibodies specific for the major birch pollen allergen Bet v 1 that cross-react with homologous food allergens. Objective: We sought to characterize the major T-cell epitopes of Bet v I and to investigate their involvement in the cellular cross-reactivity with homologous food allergens. Methods: T-cell epitope mapping of Bet v 1 was performed by testing Bet v I-specific T-cell lines derived from 57 individuals with birch pollen allergy, with overlapping peptides representing the entire allergen. T-cell lines and T-cell clones were stimulated with Bet v 1-related major allergens from apple (Mal d 1), cherry (Pru av 1), hazelnut (Cor a 1), celery (Api g 1), carrot (Dan c 1), and soybean (Gly m 4) and with peptides deduced from the C-terminal amino acid sequences of these molecules. Results: Bet v 1(142-156), positioned in the highly conserved C-terminal region of Bet v 1, was identified as the major T-cell epitope recognized by 61% of individuals. Most T lymphocytes specific for Bet v 1(142-156) were activated by one or more homologous food proteins or the respective peptides, as indicated by proliferation and cytokine production. Conclusion: The major T-cell epitope of Bet v 1, Bet v 1(142-156), plays an important role in the cellular cross-reactivity between this respiratory allergen and related food allergens. Thus T lymphocytes specific for Bet v 1(142-156) might be activated by various Bet v 1-related food allergens in vivo, even out of the pollen season.

【 授权许可】

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