Cross-reactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:121

Article

Santos, Ana Beatriz R.¹  Rocha, Gutemberg M.^2,3  Oliver, Constance^2,3  Ferriani, Virginia P. L.⁴  Lima, Rodrigo C.¹  Palma, Mirio S.⁵  Sales, Valdria S. F.⁶  Aalberse, Rob C.⁷  Chapman, Martin D.⁸  Arruda, L. Karla¹ 

[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, BR-14049900 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Cell & Mol Biol, BR-14049900 Ribeirao Preto, SP, Brazil

[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pathogen Bioagents, BR-14049900 Ribeirao Preto, SP, Brazil

[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pediat, BR-14049900 Ribeirao Preto, SP, Brazil

[5] State Univ Sao Paulo, Biosci Inst, Rio Claro, Brazil

[6] Univ Fed Rio Grande do Norte, BR-59072970 Natal, RN, Brazil

[7] Netherlands Red Cross, Blood Transfus Serv, Cent Lab, Sanquin Res, Amsterdam, Netherlands

[8] Indoor Biotechnol Inc, Charlottesville, VA USA

关键词: tropomyosin;    Ascaris lumbricoides;    periplaneta americana;    IgE cross-reactivity;    allergy;    parasites;    asthma;   

DOI  :  10.1016/j.jaci.2007.12.1147

来源: Elsevier

【 摘 要 】

Background: Evidence indicates that infection with Ascaris lumbricoides may promote development of allergy and asthma. Objective: To study the role of tropomyosin, a pan-allergen in invertebrates, in IgE responses to A lumbricoides. Methods: Recombinant A lumbricoides and Periplaneta americana tropomyosins were expressed in Pichia pastoris. Levels of IgE to tropomyosins from A lumbricoides and P americana were determined by chimeric ELISA in sera from 119 children living in a parasite-endemic area and 112 patients with cockroach allergy from the allergy clinics. Presence of tropomyosin in A lumbricoides larvae at L3 stage was evaluated by immunofluorescence using mAb IA6, directed against mite tropomyosin. Molecular modeling of P americana and A lumbricoides tropomyosins was performed by using the MODELLER program. Results: A lumbricoides tropomyosin showed 69% to 98% sequence identity to tropomyosins from other invertebrates. The predicted structure of A lumbricoides tropomyosin was similar to that of P americana tropomyosin and showed the characteristic coiled-coil structure. Strong correlation was found for IgE antibodies to tropomyosins from A lumbricoides and P americana in sera from children living in a parasite-endemic area and from patients with cockroach allergy. Larvae of A lumbricoides reacted strongly with mAb IA6. Conclusion: Tropomyosin induces IgE responses in A lumbricoides-infected children and in patients allergic to cockroach.

【 授权许可】

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