Dendritic cells and alveolarmacrophages mediate IL-13-induced airway inflammation and chemokine production

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:129

Article

Crapster-Pregont, Margaret¹  Yeo, Janice¹  Sanchez, Raquel L.¹  Kuperman, Douglas A.¹ 

[1] Northwestern Univ, Dept Med, Div Allergy Immunol, Feinberg Sch Med, Chicago, IL 60611 USA

关键词: Asthma;    airway;    macrophage;    chemokines;    inflammation;    IL-13;    IFN-gamma;    CD11b;    dendritic cell;    macrophage-derived chemokine;    thymus and activation-regulated chemokine;   

DOI  :  10.1016/j.jaci.2012.01.052

来源: Elsevier

【 摘 要 】

Background: IL-13 in the airway induces pathologies that are highly characteristic of asthma, including mucus metaplasia, airway hyperreactivity (AHR), and airway inflammation. As such, it is important to identify the IL-13-responding cell types that mediate each of the above pathologies. For example, IL-13' s effects on epithelium contribute to mucus metaplasia and AHR. IL-13' s effects on smooth muscle also contribute to AHR. However, it has been difficult to identify the cell types that mediate IL-13-induced airway inflammation. Objective: We sought to determine which cell types mediate IL-13-induced airway inflammation. Methods: We treated the airways of mice with IL-13 alone or in combination with IFN-gamma. We associated the inhibitory effect of IFN-gamma on IL-13-induced airway inflammation and chemokine production with cell types in the lung that coexpress IL-13 and IFN-gamma receptors. We then evaluated IL-13-induced responses in CD11c promoter-directed diphtheria toxin receptor-expressing mice that were depleted of both dendritic cells and alveolar macrophages and in CD11b promoter-directed diphtheria toxin receptor-expressing mice that were depleted of dendritic cells. Results: Dendritic cell and alveolar macrophage depletion protected mice from IL-13-induced airway inflammation and CCL11, CCL24, CCL22, and CCL17 chemokine production. Preferential depletion of dendritic cells protected mice from IL-13-induced airway inflammation and CCL22 and CCL17 chemokine production but not from IL-13-induced CCL11 and CCL24 chemokine production. In either case mice were not protected from IL-13-induced AHR and mucus metaplasia. Conclusions: Pulmonary dendritic cellsand alveolarmacrophages mediate IL-13-induced airway inflammation and chemokine production. (J Allergy Clin Immunol 2012; 129: 1621-7.)

【 授权许可】

Free