Increased sputum and bronchial biopsy IL-13 expression in severe asthma

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:121

Article

Saha, Shironjit K.¹  Berry, Mike A.¹  Parker, Deborah¹  Siddiqui, Salman¹  Morgan, Angela¹  May, Richard²  Monk, Phillip³  Bradding, Peter¹  Wardlaw, Andrew J.¹  Pavord, Ian D.¹  Brightling, Christopher E.¹ 

[1] Univ Hosp Leicester, Clin Sci Wing, Inst Lung Hlth, Leicester LE3 9QP, Leics, England

[2] Cambridge Antibody Technol, Cambridge, England

[3] Synairgen Res Ltd, Southampton, Hants, England

关键词: severe asthma;    IL-13;    sputum;    bronchus;    airway smooth muscle;    eosinophilia;   

DOI  :  10.1016/j.jaci.2008.01.005

来源: Elsevier

【 摘 要 】

Background: The importance of IL-13 in the asthma paradigm is supported by increased expression in human subjects, particularly in patients with mild-to-moderate asthma. However, the role of IL-13 in severe asthma needs to be further defined. Objective: We sought to assess IL-13 expression in sputum and bronchial biopsy specimens from subjects with mild-to-severe asthma. Methods: Sputum IL-13 concentrations were measured in 32 control subjects, 34 subjects with mild asthma, 21 subjects with moderate asthma, and 26 subjects with severe asthma. Enumeration of mast cells, eosinophils, and IL-13(+) cells in the bronchial submucosa and airway smooth muscle (ASM) bundle was performed in 7 control subjects, 14 subjects with mild asthma, 7 subjects with moderate asthma, and 7 subjects with severe asthma. Results: The proportion of subjects with measurable IL-13 in the sputum was increased in the mild asthma group (15/34) and severe asthma group (10/26) compared with that seen in the control group (4/32; P =.004). IL-13(+) cells were increased within the submucosa in all asthma severity groups compared with control subjects (P =.006). The number of IL-13+ cells were increased within the ASM bundle in the severe asthma group compared with that seen in the other groups (P <.05). Asthma control questionnaire scores positively correlated with sputum IL-13 concentrations (R-s = 0.35, P =.04) and mast cells in the ASM bundle (R-s = 0.7, P =.007). IL-13(+) cells within the submucosa and ASM correlated with sputum eosinophilia (R-s = 0.4, P <=.05). Conclusions: IL-13 overexpression in sputum and bronchial biopsy specimens is a feature of severe asthma.

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