Identification of cytokine-regulated genes in human leukocytes in vivo

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:105

Article

Pals, CEGM ; Verploegen, SABW ; Raaijmakers, JAM ; Lammers, JWJ ; Koenderman, L ; Coffer, PJ

关键词: differential display;    leukocyte;    neutrophil;    allergy;    priming;    GM-CSF;    5-lipoxygenase-activating protein;    PCR;   

DOI  :  10.1067/mai.2000.104382

来源: Elsevier

【 摘 要 】

Background: Human polymorphic nuclear granulocytes (PMNs) such as neutrophils and eosinophils play a critical role in mediating inflammatory responses to microbial and parasitic infections. Exposure of these leukocytes to cytokines leads to an amplification of granulocyte effector functions by a mechanism termed priming. Although many studies have investigated the effects of granulocyte priming, Little is known concerning the molecular mechanisms that Lead to this phenomenon. Objective: The purpose of this study was to identify potential markers for granulocyte priming and thus also to gain Further insight into the pathogenesis of inflammatory responses. Methods: We used a modified differential display technique, random arbitrary primed-PCR to identify genes regulated during the priming of human polymorphic nuclear granulocytes by GM-CSF in vitro. Genes identified were validated by Northern blot analysis of in vitro and in vivo primed leukocytes. Results: Several genes were identified and their expression characterized in vitro. One of these genes, 5-lipoxygenase-activating protein, was also found to be up-regulated in leukocytes isolated after allergen challenge of allergic asthmatic patients. Conclusion: The use of differential display technology is a rapid and effective means of identifying genes whose expression is regulated by priming in vitro and in vivo. Further analysis will lead to a better understanding of the priming phenotype and may provide further insight into the pathologic mechanisms of inflammatory processes.

【 授权许可】

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