Basophils, high-affinity IgE receptors, and CCL2 in human anaphylaxis

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:140

Article

Korosec, Peter¹  Turner, Paul J.^2,3,4  Silar, Mira¹  Kopac, Peter¹  Kosnik, Mitja¹  Gibbs, Bernhard F.⁵  Shamji, Mohamed H.^3,4,6  Custovic, Adnan^2,3,4  Rijavec, Matija¹ 

[1] Univ Hosp Resp & Allerg Dis, Golnik, Slovenia

[2] Imperial Coll London, Sect Paediat, London, England

[3] Imperial Coll London, MRC, London, England

[4] Imperial Coll London, Asthma UK Ctr Allerg Mech Asthma, London, England

[5] Univ Kent, Medway Sch Pharm, Chatham, Kent, England

[6] Imperial Coll London, Natl Heart & Lung Inst, Sect Allergy & Clin Immunol, London, England

关键词: Anaphylaxis;    basophils;    CD63 activation;    Fc epsilon RI expression;    CCL2;    serum tryptase;   

DOI  :  10.1016/j.jaci.2016.12.989

来源: Elsevier

【 摘 要 】

Background: The role of basophils in anaphylaxis is unclear. Objective: We sought to investigate whether basophils have an important role in human anaphylaxis. Methods: In an emergency department study we recruited 31 patients with acute anaphylaxis, predominantly to Hymenoptera venom. We measured expression of basophil activation markers (CD63 and CD203c); the absolute number of circulating basophils; whole-blood FCER1A, carboxypeptidase A3 (CPA3), and L-histidine decarboxylase (HDC) gene expression; and serum markers (CCL2, CCL5, CCL11, IL-3, and thymic stromal lymphopoietin) at 3 time points (ie, during the anaphylactic episode and in convalescent samples 7 and 30 days later). We recruited 134 patients with Hymenoptera allergy and 76 healthy control subjects for comparison. We then investigated whether the changes observed during venom-related anaphylaxis also occur during allergic reactions to food in 22 patients with peanut allergy undergoing double-blind, placebo-controlled food challenge to peanut. Results: The number of circulating basophils was significantly lower during anaphylaxis (median, 3.5 cells/mu L) than 7 and 30 days later (17.5 and 24.7 cells/mu L, P < .0001) and compared with those in patients with venom allergy and healthy control subjects (21 and 23.4 cells/mu L, P < .0001). FCER1A expression during anaphylaxis was also significantly lower than in convalescent samples (P <= .002) and control subjects with venom allergy (P < .0001). CCL2 levels (but not those of other serum markers) were significantly higher during anaphylaxis (median, 658 pg/mL) than in convalescent samples (314 and 311 pg/mL at 7 and 30 days, P < .001). Peanut-induced allergic reactions resulted in a significant decrease in circulating basophil counts compared with those in prechallenge samples (P = .016), a decrease in FCER1A expression (P = .007), and an increase in CCL2 levels (P = .003). Conclusions: Our findings imply an important and specific role for basophils in the pathophysiology of human anaphylaxis.

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