MODULATION BY INTERLEUKIN-4 OF CYTOKINE RELEASE FROM MONONUCLEAR PHAGOCYTES IN ASTHMA

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:94

Article

CHANEZ, P ; VIGNOLA, AM ; PAULEUGENE, N ; DUGAS, B ; GODARD, P ; MICHEL, FB ; BOUSQUET, J

关键词: MONOCYTES;    ALVEOLAR MACROPHAGES;    ASTHMA;    IL-1;    TNF-ALPHA;    IL-6;    IL-4;   

DOI  :  10.1016/0091-6749(94)90118-X

来源: Elsevier

【 摘 要 】

Background: Interleukin (IL)-4 is involved in IgE upregulation and downregulates cytokine release by mononuclear phagocytes. Mononuclear cells release greater amounts of IL-1, tumor necrosis factor-alpha, and IL-6 in patients with asthma than in control subjects, but the effect of IL-4 on cells from patients with asthma is unknown. The effects of IL-4 on the release of IL-1, tumor necrosis factor-alpha, and IL-6 by monocytes and alveolar macrophages were compared in 19 patients with asthma and 18 control subjects. Methods: The release of IL-1, tumor necrosis factor-alpha, and IL-6 from unstimulated and lipopolysaccharide stimulated monocytes and alveolar macrophages was measured by ELISA. The effect of 30 U of IL-4 on the release of these cytokines was studied. Results: Lipopolysaccharide-stimulated monocytes released significantly fewer cytokines in patients with asthma than in control subjects. IL-4 significantly inhibited cytokine release by monocytes of both groups. Unstimulated alveolar macrophages from patients with asthma released more cytokines than those of control subjects. Lipopolysaccharide induced a significantly greater increase in cytokine release in alveolar macrophages of control subjects n comparison with asthmatic subjects. IL-4 abolished the release of cytokines in alveolar macrophages from control subjects and had a minimal inhibitory effect on alveolar macrophages from patients with asthma. Conclusions: Alveolar macrophages from patients with asthma are hyperreactive but less prone to lipopolysaccharide stimulation and IL-4-downregulation than those from normal subjects.

【 授权许可】

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