IL-13 involvement in eosinophilic esophagitis: Transcriptome analysis and reversibility with glucocorticoids

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:120

Article

Blanchard, Carine¹  Mingler, Melissa K.¹  Vicario, Maria^1,2  Abonia, J. Pablo¹  Wu, Yi Ying¹  Lu, Thomas X.¹  Collins, Margaret H.³  Putnam, Philip E.⁴  Wells, Susanne I.⁵  Rothenberg, Marc E.¹ 

[1] Cincinnati Childrens Hosp Med Ctr, Dept Pediat, Div Allergy & Immunol, Cincinnati, OH 45229 USA

[2] Univ Autonoma Barcelona, Hosp Univ Vall Hebron, Dept Gastroenterol, Digest Dis Res Unit, E-08193 Barcelona, Spain

[3] Univ Cincinnati, Coll Med, Cincinnati Childrens Hosp & Med Ctr, Dept Pediat,Div Pathol & Lab Med, Cincinnati, OH USA

[4] Univ Cincinnati, Coll Med, Cincinnati Childrens Hosp & Med Ctr, Dept Pediat,Div Gastroenterol Hepatol & Nutr, Cincinnati, OH USA

[5] Univ Cincinnati, Coll Med, Cincinnati Childrens Hosp & Med Ctr, Dept Pediat,Div Hematol Oncol, Cincinnati, OH USA

关键词: IL-13;    eosinophilic esophagitis;    eotaxin-3;    cytokines;    esophageal epithelial cells;    keratinocytes;   

DOI  :  10.1016/j.jaci.2007.10.024

来源: Elsevier

【 摘 要 】

Background: Eosinophilic esophagitis (EE) is an emerging worldwide disease that mimics gastroesophageal reflux disease. Early studies have established that esophageal eosinophilia occurs in association with T(H)2 allergic responses, and we recently identified an EE-specific esophageal transcriptome that included eotaxin-3. Objective: We sought to determine the mechanism by which this TH2 response leads to EE. Methods: Real-time PCR and microarray analysis were performed on RNA extracted from esophageal biopsy specimens and primary esophageal epithelial cell cultures stimulated with IL-13 (0-100 ng/mL). Transient transfections in esophageal cell lines were performed with plasmids containing the luciferase gene driven by eotaxin-3 promoter fragments and modified forms of signal transducer and activator of transcription 6. Results: The IL-13 mRNA level was markedly increased (16-fold) in esophageal biopsy specimens from patients with EE compared with those from healthy individuals. Furthermore, IL-13 treatment of primary esophageal epithelial cells was sufficient to induce a global-expression transcript profile that remarkably overlapped with the EE-specific esophageal transcriptome. In addition, esophageal epithelial cells markedly produce eotaxin-3 after IL-13 stimulation through a transcriptional mechanism dependent on signal transducer and activator of transcription 6. Lastly, increased IL-13 mRNA levels and the EE transcriptome were largely reversible with glucocorticoid treatment in vivo. Conclusions: Taken together, we propose that the pathogenesis of EE is mediated by an IL-13-stimulated keratinocyte-derived transcriptome that is largely reversible with corticosteroid treatment. Furthermore, we identify an in vivo IL-13-induced transcriptome that has potential utility for target assessment after anti-IL-13 therapeutics. Clinical implications: IL-13-induced pathways and genes are fundamental processes in the cause and manifestations of EE; as such, therapeutic agents that interfere with IL-13 might be particularly useful for disease treatment.

【 授权许可】

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