ACTIVATION OF CD4+ T-CELLS, INCREASED T(H2)-TYPE CYTOKINE MESSENGER-RNA EXPRESSION, AND EOSINOPHIL RECRUITMENT IN BRONCHOALVEOLAR LAVAGE AFTER ALLERGEN INHALATION CHALLENGE IN PATIENTS WITH ATOPIC ASTHMA

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:92

Article

ROBINSON, D ; HAMID, Q ; BENTLEY, A ; YING, S ; KAY, AB ; DURHAM, SR

关键词: T(H2) CELLS;    EOSINOPHILS;    IN-SITU HYBRIDIZATION;    FLOW CYTOMETRY;    LATE ASTHMATIC RESPONSE;   

DOI  :  10.1016/0091-6749(93)90175-F

来源: Elsevier

【 摘 要 】

Background: We have examined whether the local eosinophilia provoked by inhalational allergen challenge of patients with atopic asthma is associated with the appearance, in vivo, of activated T(H2)-type T helper lymphocytes Methods: Fifteen patients with atopic asthma had bronchial wash and bronchoalveolar lavage (BAL) 24 hours after allergen or diluent challenge separated by at least 21 days. Results: There was an increase in eosinophils in both bronchial wash (p = 0. 01) and BAL (p = 0. 02) after allergen challenge but not after diluent challenge. Activation of CD4 + BAL T cells was suggested by an increase in the expression of CD25 shown by flow cytometry after allergen challenge, when compared with diluent (p = 0 02). There was no evidence of activation of CD8 T cells. By in situ hybridization after allergen challenge as compared with diluent, increases were shown in the numbers of cells expressing mRNA for interleukin-4 (IL-4) (p = 0. 005), IL-5 (p = 0. 01), and granulocyte-macrophage colony-stimulating factor (p = 0.03) but not IL-3, IL-2, or interferon-gamma. In situ hybridization of BAL cells after immunomagnetic separation of CD2-positive and CD2-negative cell populations showed that IL-4 and IL-5 mRNAs were associated with T lymphocytes after allergen challenge. BAL and bronchial wash eosinophilia closely correlated with maximal late fall in forced expiratory volume in 1 second after allergen challenge. Conclusion: Cytokines produced by activated T(H2)-type CD4 + T cells in the airway may contribute to late asthmatic responses by mechanisms that include eosinophil accumulation.

【 授权许可】

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