Allergic dysregulation and hyperimmuno-globulinemia E in Foxp3 mutant mice

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:116

Article

Lin, W ; Truong, N ; Grossman, WJ ; Haribhai, D ; Williams, CB ; Wang, JF ; Martín, MG ; Chatila, TA

关键词: Foxp3;    dysregulation polyendocrinopathy enteropathy-X-linked;    allergic inflammation;    regulatory T cells;    signal transducer and activator of transcription 6;   

DOI  :  10.1016/j.jaci.2005.08.046

来源: Elsevier

【 摘 要 】

Background: Regulatory T cells have been proposed to play an important role in regulating allergic inflammation. The transcription factor Foxp3 is a master switch gene that controls the development and function of natural and adaptive CD4(+)CD25(+) regulatory T (T-R) cells. In human subjects loss-of-function Foxp3 mutations trigger lymphoproliferation, autoimmunity, and intense allergic inflammation in a disease termed immune dysregulation polyendocrinopathy enteropathy-X-linked syndrome. Objective: We sought to examine the evolution and attributes of allergic inflammation in mice with a targeted loss-of-function mutation in the murine Foxp3 gene that recapitulates a known disease-causing human Foxp3 mutation. Methods: Foxp3 mutant mice were generated by means of knock-in mutagenesis and were analyzed for histologic, immunologic, and hematologic abnormalities. The role of signal transducer and activator of transcription 6 (Stat6) in disease pathogenesis was analyzed by using Stat6 and Foxp3 double-mutant mice. Results: Foxp3 mutant mice developed an intense multiorgan inflammatory response associated with allergic airway inflammation, a striking hyperimmunoglobulinemia E, eosinophilia, and dysregulated T(H)1 and T(H)2 cytokine production in the absence of overt T(H)2 skewing. Concurrent Stat6 deficiency reversed the hyperimmunoglobulinemia E and eosinophilia and delayed mortality, which is consistent with a pathogenic role for allergic inflammation in Foxp3 deficiency. Conclusion: Allergic dysregulation is a common and fundamental consequence of loss of CD4(+)CD25(+) T-R cells caused by Foxp3 deficiency in different species. Abnormalities affecting TR cells might contribute to a variety of allergic diseases.

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