Combination treatment with omalizumab and rush immunotherapy for ragweed-induced allergic rhinitis: Inhibition of IgE-facilitated allergen binding

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:120

Article

Klunker, Sven ; Saggar, Lavina R. ; Seyfert-Margolis, Vicki ; Asare, Adam L. ; Casale, Thomas B. ; Durham, Stephen R. ; Francis, James N.

关键词: allergy;    immunotherapy;    omalizumab;    anti-IgE;    IgEfacilitated allergen binding;    ragweed;   

DOI  :  10.1016/j.jaci.2007.05.034

来源: Elsevier

【 摘 要 】

Background: The combination of anti-IgE (omalizumab) therapy with ragweed injection immunotherapy for seasonal allergic rhinitis results in a significant reduction in systemic side effects and enhanced efficacy compared with immunotherapy alone. One proposed mechanism of immunotherapy is to induce regulatory antibodies that inhibit facilitated antigen presentation. Objectives: We sought to determine whether the combination protocol has a cumulative effect on inhibition of facilitated antigen presentation both during and after discontinuation of treatment. Methods: Ragweed allergen immunotherapy with and without omalizumab therapy was tested in a 4-arm, double-blind, placebo-controlled study. Flow cytometry was used to detect serum inhibitory activity for IgE-facilitated CD23-dependent allergen binding to B cells as a surrogate marker for facilitated antigen presentation. Serum ragweed-specific IgG4 was measured by means of ELISA. Results: Immunotherapy alone resulted in partial inhibition of allergen-IgE binding after 5 to 19 weeks of treatment compared with baseline (P <.01). Complete inhibition of allergen-specific IgE binding was observed in both treatment groups receiving omalizumab (P <.001). Allergen-specific IgG4 levels were only increased after immunotherapy (P <.05), both in the presence and absence of anti-IgE treatment. Combined treatment resulted in the induction of long-lasting inhibitory antibody function for up to 42 weeks compared with either treatment alone. Conclusion: Ragweed immunotherapy induced serum regulatory antibodies that partially blocked binding of allergen-IgE complexes to B cells. Additional treatment with anti-IgE, by directly blocking IgE binding to CD23, completely inhibited allergen-IgE binding. Clinical implications: The combination of ragweed immunotherapy and anti-IgE resulted in prolonged inhibition of allergen-IgE binding compared with either treatment alone, events that might contribute to enhanced efficacy.

【 授权许可】

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