INVOLVEMENT OF IMMUNOLOGICAL MECHANISMS IN A GUINEA-PIG MODEL OF WESTERN RED CEDAR ASTHMA

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:93

Article

SALARI, H ; HOWARD, S ; CHAN, H ; DRYDEN, P ; CHANYEUNG, M

关键词: OCCUPATIONAL ASTHMA;    GUINEA PIG;    PLICATIC ACID;    HISTAMINE LEUKOTRIENES;    PROSTAGLANDIN;    IGG(1);   

DOI  :  10.1016/0091-6749(94)90381-6

来源: Elsevier

【 摘 要 】

Western red cedar asthma is the most common form of occupational asthma in the Pacific Northwest. Plicatic acid (PA) is the chemical component of western red cedar that causes asthma. The role of immunologic processes involved in the PA-induced asthmatic reaction has not been established. To characterize the mechanisms of PA-induced asthmatic reaction, guinea pigs were sensitized to PA through biweekly injection of PA-ovalbumin conjugate with aluminum hydroxide as an adjuvant for a period of 6 months. Specific IgG(1) antibodies to PA were detected in the blood 3 months after sensitization of animals. The level of specific IgG(1) antibodies to ovalbumin after 6 months was about two times the level of specific IgG(1) to PA. At 6 months, tracheal tissue from PA-ovalbumin-sensitized guinea pigs contracted after exposure to either PA or ovalbumin in vitro. The degree of contraction induced by PA was two to three times less than the contraction induced by ovalbumin. PA caused histamine prostaglandin D-2 and leukotriene D-4 release from both lung mast cells and blood basophils. The amount of histamine and eicosanoids released by PA was also two to three times less than the amount of mediators released by ovalbumin. When the trachea of normal guinea pigs was passively sensitized with serum from PA-ovalbumin-sensitized guinea pigs, it contracted in response to PA or ovalbumin in an organ bath. When the serum of PA-ovalbumin-sensitized guinea pigs was depleted of immunoglobulins and then used for passive sensitization of normal trachea, no contraction was observed when challenged with PA, suggesting that IgG(1) antibodies mediate the tracheal reaction to PA. The results demonstrated that PA-induced reaction in guinea pigs is an immunologic process that involves IgG(1) antibodies.

【 授权许可】

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