Comparison of CD8+ T-cell subsets in HIV-infected rapid progressor children versus non-rapid progressor children

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:108

Article

Paul, ME ; Shearer, WT ; Kozinetz, CA ; Lewis, DE

关键词: pediatric HIV-1 infection;    CD4(+) T cells;    CD8(+) T cells;    CD8(+) T-cell subsets;    HIV disease progression;   

DOI  :  10.1067/mai.2001.117179

来源: Elsevier

【 摘 要 】

Background: CD8(+) T-cell subsets have not been adequately described in HIV-infected (HIV+) children classified with respect to disease progression as rapid-progressors (RPs) and non-rapid progressors (non-RPs). Objective: The purpose of this investigation was to determine the distribution of CD8(+) T-cell subsets in HIV+ children and correlate the findings with degree of immunosuppression and HIV viral burden. Methods: By means of 3-color flow cytometry, percentages of CD38(+)DR(+), CD28(+), and CD57(+) CD8(+) T-cell subsets were examined in RP (n=15) and non-RP (n=36) HIV+ children and in HIV- exposed but uninfected (n=11) and HIV-unexposed (n=8) children. The CD8(+) T-cell subsets were correlated with mean CD4(+) T-cell percentages and HIV RNA levels. Analysis of covariance was used for group comparisons for the control of the covariate of age. Results: The HIV-exposed and HIV-unexposed controls were not different from each other in CD8(+) T-cell subset percentages, except that the DR(-)CD38(+)CD8(+) T-cell percentages were higher in the exposed controls than in the unexposed controls. RPs had a higher mean percentage of DR(+)CD38(+)CD8(+) T cells than non-RPs and both control groups, and RPs had higher viremia than non-RPs. CD38(+)CD8(+) T-cell percentages did not correlate with viral burden as it has been seen to do in HIV+ adults. Percentages of CD28(+)CD8(+) T cells were lower in HIV-infected children than in controls. There was a positive correlation of percentage of CD28(+)CD57(-)CD8(+) T cells with CD4(+) T-cell percentages in each HIV-infected group. Conclusion: CD8(+) T cells become activated (dual expression of DR and CD38) and lose CD28, some acquiring CD57, in relation to rapidity of disease progression in pediatric HIV infection.

【 授权许可】

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