AEROSOLIZED ANTIGEN EXPOSURE WITHOUT ADJUVANT CAUSES INCREASED IGE PRODUCTION AND INCREASED AIRWAY RESPONSIVENESS IN THE MOUSE

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:89

Article

RENZ, H ; SMITH, HR ; HENSON, JE ; RAY, BS ; IRVIN, CG ; GELFAND, EW

关键词: ALTERED AIRWAY RESPONSIVENESS;    IGE;    MOUSE;    INVIVO LUNG FUNCTION;    OVALBUMIN;   

DOI  :  10.1016/0091-6749(92)90296-E

来源: Elsevier

【 摘 要 】

Inhalation of an antigen, ovalbumin (OVA), in the absence of adjuvant has been demonstrated to induce an immune response that is associated with increase airway responsiveness. Determination of OVA-specific serum IgE and IgG antibody responses revealed an early increase in antibody titers that were initially restricted to the IgE class. Subsequently, IgG antibody titers increased and IgE antibody plateaued. Furthermore, we observed a tenfold increase in the number of lymphocytes caused by a predominant expansion of CD3+ T cells in the peribronchial-associated lymph modes (PBLNs) of sensitized animals compared with the numbers of cells in control animals or in the gut-associated lymphoid tissue. The sensitized animals demonstrated an increase in airway responsiveness to intravenous methacholine challenge. Analysis of in vitro immunoglobulin production by spleen mononuclear cells revealed increased spontaneous IgE production that was more than fourfold enhanced in the presence of OVA, but IgG production was not increased. Spleen and PBLN lymphocytes, but not lymphocytes from gut-draining lymp nodes, demonstrated a proliferative response to OVA. Control animals exhibited no proliferative response to OVA. Histopathologic examination of the sensitized lung revealed an absence of acute inflammatory cells (e.g., neutrophils and macrophages), lymphocytes, or monocytes at the time of the increased airway hyperresponsiveness. These data indicate that, after sensitization of mice by inhalation of antigen, the animals develop a specific IgE antibody response, expansion of PBLN lymphocyte numbers, and increased airway hyperresponsiveness in the absence of signs of airway inflammation.

【 授权许可】

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