Functional expression of chemokine receptor 2 by normal human eosinophils

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:108

Article

Dunzendorfer, S ; Kaneider, NC ; Kaser, A ; Woell, E ; Frade, JMR ; Mellado, M ; Martínez-Alonso, C ; Wiedermann, CJ

关键词: eosinophils;    eosinophilic infiltration;    chemokines;    chemotaxis;    receptor;    signal transduction;   

DOI  :  10.1067/mai.2001.118518

来源: Elsevier

【 摘 要 】

Background: Within the granulocytes, the CC chemokines preferentially activate basophils and eosinophils on binding to chemokine receptors (CCRs). In vivo administration of neutralizing anti-monocyte chemoattractant protein 1 (MCP-1) antibodies can block accumulation of eosinophils in the lungs of antigen-challenged animals. Objective: We studied a panel of chemokines for chemotactic activity in normal human eosinophils from healthy donors with a special focus on MCP-1, identified the respective receptor required for the biological response of eosinophils, and investigated mediators used for signal transduction. Methods: Cells were enriched by magnetic cell sorting. Receptor expression in cosinophils was shown by RT-PCR and fluorescence-activated cell sorting. The biological response was tested in chemotaxis and calcium mobilization assays. Results: Eosinophils have detectable mRNA for CCR2, and the receptor protein is expressed on cell surfaces. MCP-1 induces chemotaxis and calcium mobilization in eosinophils. The chemotactic activity of MCP-1 revealed a double-peaked dose-response curve; one of the peaks is abolished by addition of a blocking antibody to CCR2, but it is insensitive to blocking of CCR1 or CCR3. Specific enzyme inhibitors ruled out signaling characteristics of CCR2 in eosinophils. Conclusion: Normal human eosinophils express functional CCR2 on cell surfaces.

【 授权许可】

Free