Platelet-derived growth factor is involved in the augmentation of airway responsiveness through remodeling of airways in diesel exhaust particulate-treated mice

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:107

Article

Yamashita, N ; Sekine, K ; Miyasaka, T ; Kawashima, R ; Nakajima, Y ; Nakano, J ; Yamamoto, T ; Horiuchi, T ; Hirai, K ; Ohta, K

关键词: asthma;    airway hyperresponsiveness;    platelet-derived growth factor;    airway remodeling;    diesel exhaust particulate;    acetylcholine;   

DOI  :  10.1067/mai.2001.111433

来源: Elsevier

【 摘 要 】

Background: Thickening of the region adjacent to the basement membrane is a key component of the remodeling of the asthmatic airway and is caused by collagen deposition in the region. Objective: We sought to clarify the role of platelet-derived growth factor (PDGF), a competence factor of fibroblast, in the enhanced airway responsiveness and remodeling in a murine model. Methods: Diesel exhaust particulates (DEPs) were administered intranasally every other day for 2 weeks with or without anti-PDGF-beta neutralizing antibody or goat IgG. Pulmonary function was then analyzed by using whole-body plethysmography before and after acetylcholine inhalation. Results: Anti-PDGF-beta neutralizing antibody significantly inhibited both the elevation of airway resistance elicited by 1.25 and 2.5 mg/mL acetylcholine and the increase in the airway wall thickening induced by DEPs. In addition, bronchoalveolar lavage fluid cell analysis revealed that anti-PDCF-beta neutralizing antibody did not affect cellular infiltration at the airways. Conclusion: PDGF plays an important role in the process of remodeling brought about by DEP exposure in mice.

【 授权许可】

Free