Staphylococcal protein A-formulated immune complexes suppress enterotoxin-induced cellular responses in nasal polyps

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:136

Article

Okano, Mitsuhiro¹  Fujiwara, Tazuko¹  Kariya, Shin¹  Haruna, Takenori¹  Higaki, Takaya¹  Noyama, Yasuyuki¹  Makihara, Sei-ichiro²  Kanai, Kengo³  Nishizaki, Kazunori¹ 

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Otolaryngol Head & Neck Surg, Okayama 7008558, Japan

[2] Kagawa Rosai Hosp, Dept Otorhinolaryngol, Marugame, Kagawa, Japan

[3] Kagawa Prefectural Cent Hosp, Dept Otorhinolaryngol, Takamatsu, Kagawa, Japan

关键词: Chronic rhinosinusitis with nasal polyps;    cytokines;    enterotoxins;    immune complexes;    immunoglobulins;    immune evasion;    Staphylococcus aureus;    staphylococcal protein A;   

DOI  :  10.1016/j.jaci.2014.10.058

来源: Elsevier

【 摘 要 】

Background: Recent studies have revealed that Staphylococcus aureus and its components participate in the pathogenesis of eosinophilic airway diseases, such as chronic rhinosinusitis with nasal polyps. Objective: We sought to determine whether staphylococcal protein A (SpA) from S aureus regulated cellular responses in nasal polyps, especially when coupled to immunoglobulins in immune complexes (ICs). Methods: Dispersed nasal polyp cells (DNPCs) or peripheral blood monocytes were cultured in vitro with SpA in the presence or absence of IgG, and IL-5, IL-13, IFN-gamma, IL-17A, and IL-10 levels were measured in the supernatants. The effect of SpA exposure on staphylococcal enterotoxin B-induced cytokine production by DNPCs in the presence and absence of IgG, IgA, and autologous serum was also examined. Results: Exposure to SpA induced DNPCs to produce significantly higher IL-10, IL-13, and IL-17A levels than DNPCs without SpA, although the magnitude of the IL-17A increase was less than that of IL-10 and IL-13. SpA induced IL-10 production mainly from adherent DNPCs, and this was significantly enhanced in the presence of IgG; similar results were observed in peripheral blood monocytes. IC formation between SpA and IgG (SpA-IgG ICs) was confirmed by using native polyacrylamide gel electrophoresis. SpA-IgG ICs, but not SpA alone, almost completely suppressed staphylococcal enterotoxin B-induced IL-5, IL-13, IFN-gamma, and IL-17A production by DNPCs; similar inhibition was observed in DNPCs treated with SpA in the presence of either IgA or autologous serum. Conclusions: Our results suggest that SpA can regulate the pathogenesis of enterotoxin-induced inflammation in patients with chronic rhinosinusitis with nasal polyps through coupling to immunoglobulins.

【 授权许可】

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