Cockroach allergen component analysis of children with or without asthma and rhinitis in an inner-city birth cohort

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:144

Article

Pomes, Anna¹  Glesner, Jill¹  Calatroni, Agustin²  Visness, Cindy M.²  Wood, Robert A.^3,4  O'Connor, George T.⁵  Kattan, Meyer⁶  Bacharier, Leonard B.⁷  Wheatley, Lisa M.⁸  Gem, James E.^9,10  Busse, William W.^9,10  Wood, R.¹¹  Matsui, E.¹¹  Lederman, H.¹¹  Witter, F.¹¹  Leimenstoll, S.¹¹  Scott, D.¹¹  Cootauco, M.¹¹  Jones, P.¹¹  O'Connor, G.¹²  Cruikshank, W.¹²  Sandel, M.¹²  Lee-Parritz, A.¹²  Jordan, C.¹²  Gjerasi, E.¹²  Price-Johnson, P.¹²  Gagalis, L.¹²  Wang, L.¹²  Gonzalez, N.¹²  Tuzova, M.¹²  Gold, D.¹³  Wright, R.¹³  Kaftan, M.¹⁴  Lamm, C.¹⁴  Whitney, N.¹⁴  Yaniv, P.¹⁴  Pierce, M.¹⁴  Sampson, H.¹⁵  Sperling, R.¹⁵  Rivers, N.¹⁵  Bloomberg, G.¹⁶  Bacharier, L.¹⁶  Sadovsky, Y.¹⁶  Tesson, E.¹⁶  Koerkenmeier, C.¹⁶  Sharp, R.¹⁶  Ray, K.¹⁶  Durrange, J.¹⁶  Bauer, I¹⁶  Freie, A.¹⁶  Morgan, V¹⁶  Visness, C.¹⁷  Zook, P.¹⁷  Yaeger, M.¹⁷  Martin, J.¹⁷  Calatroni, A.¹⁷  Jaffee, K.¹⁷  Taylor, W.¹⁷  Budrevich, R.¹⁷  Mitchell, H.¹⁷  Busse, W.¹⁸  Gem, J.¹⁸  Heinritz, P.¹⁸  Sorkness, C.¹⁸  Hernandez, K.¹⁸  Bochkov, Y.¹⁸  Grindle, K.¹⁸  Dresen, A.¹⁸  Pappas, T.¹⁸  Renneberg, M.¹⁸  Stoffel, B.¹⁸  Gergen, P.^18,19  Togias, A.¹⁹  Smartt, E.¹⁹  Thompson, K.¹⁹ 

[1] Indoor Biotechnol Inc, Basic Res, Charlottesville, VA 22903 USA

[2] Rho Fed Syst Div, Chapel Hill, NC USA

[3] Johns Hopkins Univ, Dept Pediat, Baltimore, MD 21218 USA

[4] Johns Hopkins Univ, Dept Allergy & Immunol, Baltimore, MD 21218 USA

[5] Boston Univ, Sch Med, Dept Medicne, Boston, MA 02118 USA

[6] Columbia Univ, Dept Pediat, New York, NY 10027 USA

[7] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA

[8] NIAID, Div Allergy Immunol & Transplantat, 9000 Rockville Pike, Bethesda, MD 20892 USA

[9] Univ Wisconsin, Dept Pediat, Madison, WI USA

[10] Univ Wisconsin, Dept Med, Madison, WI USA

[11] Johns Hopkins Univ, Baltimore, MD USA

[12] Boston Univ, Sch Med, Boston, MA 02118 USA

[13] Harvard Med Sch, Boston, MA 02115 USA

[14] Columbia Univ, New York, NY USA

[15] Mt Sinai Sch Med, New York, NY USA

[16] Washington Univ, Sch Med, St Louis, MO USA

[17] Rho Inc, Stat & Clin Coordinating Ctr, Chapel Hill, NC USA

[18] Univ Wisconsin, Sci Coordinat & Adm Ctr, Madison, WI USA

[19] NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA

关键词: Cockroach allergy;    cockroach allergen components;    asthma;    rhinitis;    diagnosis;    immunotherapy;   

DOI  :  10.1016/j.jaci.2019.05.036

来源: Elsevier

【 摘 要 】

Background: Cockroach is one of the most important sources of indoor allergens and can lead to IgE sensitization and development of rhinitis and asthma. Objective: We sought to perform a cockroach allergen component analysis to determine the allergens and antibody levels and patterns of sensitization associated with asthma and rhinitis. Methods: Antibody (IgE, IgG, and IgG(4)) levels to total cockroach and 8 cockroach allergens were determined in 2 groups of cockroach-sensitized 10-year-old children with (n = 19) or without (n = 28) asthma and rhinitis. Allergen-specific antibody levels were measured in streptavidin ImmunoCAPs loaded with each of the recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9, and 11, and total cockroach-specific IgE levels were measured with the i6 ImmunoCAP. Results: IgE antibody levels to cockroach allergens and extract, but not IgG or IgG(4) antibody levels, differed between subjects with and without asthma and rhinitis. Specifically, recognition of more cockroach allergens with higher allergen-specific IgE levels was associated with disease. Variable patterns of sensitization with no immunodominant allergens were found in both groups. There was a good correlation between the sum of allergen-specific IgE and total cockroach IgE levels (r = 0.86, P < .001). Conclusions: Component analysis of 8 cockroach allergens revealed significant differences in IgE reactivity associated with the presence of asthma and rhinitis. Allergen-specific IgE titers and sensitization profiles were associated with asthma and rhinitis.

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