IGE REGULATION AND LYMPHOKINE PATTERNS IN AGING HUMANS

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:90

Article

ALRAYES, H ; PACHAS, W ; MIRZA, N ; AHERN, DJ ; GEHA, RS ; VERCELLI, D

关键词: IGE;    AGING;    LYMPHOKINES;    IL-4;    IFN-GAMMA;    CALCIUM FLUXES;   

DOI  :  10.1016/0091-6749(92)90136-P

来源: Elsevier

【 摘 要 】

IgE production declines with age, and allergic symptoms tend to improve. Aging therefore represents an in vivo model to study IgE regulation. We compared IgE production in older (greater-than-or-equal-to 60 years old) and young (15 to 30 years old) nonatopic individuals. Addition of exogenous interleukin-4 (IL-4) to mononuclear cells from older and young subjects induced equivalent amounts of IgE, indicating that IL-4 responsiveness is preserved in aging. After surface receptor stimulation with concanavalin A, IL-4 production by mononuclear cells from older subjects was approximately 50% as compared with the young, whereas interferon-gamma (IFN-gamma) production was reduced threefold (p = 0.008). By contrast, stimulation with phorbol esters and ionophore, which bypass surface receptor signaling, induced comparable amounts of IL-4 and IFN-gamma in older and young subjects. These data point to an impairment in T-cell membrane signal transduction in older individuals. This hypothesis was directly confirmed by showing that Ca+2 fluxes after CD3 crosslinking were significantly (p = 0.014) decreased in the older population. Our findings altogether suggest that an age-dependent T-cell activation defect may result in decreased availability of IL-4 and in the waning of IgE responses.

【 授权许可】

Free