Chemokine production by the BEAS-2B human bronchial epithelial cells:: Differential regulation of eotaxin, IL-8, and RANTES by TH2-and TH1-derived cytokines

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:105

Article

Fujisawa, T ; Kato, Y ; Atsuta, J ; Terada, A ; Iguchi, K ; Kamiya, H ; Yamada, H ; Nakajima, T ; Miyamasu, M ; Hirai, K

关键词: chemokines;    bronchial epithelial cells;    eotaxin;    RANTES;    IFN-gamma;    IL-4;    IL-13;    IL-8;   

DOI  :  10.1016/S0091-6749(00)90187-8

来源: Elsevier

【 摘 要 】

Background: Bronchial epithelial cells produce many types of chemokines and may contribute to lung inflammation by recruiting inflammatory cells. The CC chemokine eotaxin is a potent, eosinophil-specific chemoattractant that has been detected in the bronchial epithelium of patients with asthma. Objectives: The aim of this study was to investigate the regulatory mechanisms of chemokine production from bronchial epithelium by inflammatory cytokines, especially T(H)2- and T(H)1-derived cytokines, in bronchial asthma. Methods: BEAS-2B human bronchial epithelial cells were cultured with TNF-alpha, IL-4, IL-13, and IFN-gamma alone or in combination, after which supernatants were assayed for eotaxin, IL-8, and RANTES proteins with ELISA. Reverse transcription-PCR was also performed. Results: TNF-alpha induced production of eotaxin, IL-8, and RANTES in a concentration-dependent manner Both IL-4 and IL-13 synergistically enhanced TNF-alpha-induced eotaxin production, whereas IL-8 plarluction induced by TNF-alpha was significantly down-regulated by the Tea-derived cytokines. IFN-gamma, a T(H)1 cytokine, counteracted the enhancing effects of IL-4 and IL-13 on eotaxin production. RANTES production by TNF-alpha was not affected by IL-4 and IL-13 but was markedly enhanced by IFN-gamma. Conclusions:These results suggest that T(H)2 cytokines are involved in preferential recruitment of eosinophils in bronchial asthma by enhancing eotaxin and reducing IL-8 production from bronchial epithelial cells and that T(H)1 cytokines counteract the effects of T(H)2 cytokines by reducing eotaxin production.

【 授权许可】

Free