Staphylococcus aureus internalization in mast cells in nasal polyps: Characterization of interactions and potential mechanisms

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:145

Article

Hayes, Stephen M.^1,3,4,5,6  Biggs, Timothy C.^1,3,4,5,6  Goldie, Simon P.^1,3,4,5,6  Harries, Philip G.⁵  Walls, Andrew F.¹  Allan, Raymond N.^2,6  Pender, Sylvia L. F.¹  Salib, Rami J.^1,3,4,5,6 

[1] Univ Southampton, Fac Med, Sch Clin & Expt Sci, Southampton, Hants, England

[2] Univ Southampton, Fac Environm & Life Sci, Dept Biol Sci, Southampton, Hants, England

[3] Univ Southampton, Southampton NIHR Resp Biomed Res Ctr, Southampton, Hants, England

[4] Univ Hosp Southampton NHS Fdn Trust, Southampton, Hants, England

[5] Univ Hosp Southampton NHS Fdn Trust, Dept Otorhinolaryngol Head & Neck Surg, Tremona Rd, Southampton SO16 6YD, Hants, England

[6] Univ Hosp Southampton NHS Fdn Trust, NIHR Wellcome Trust Clin Res Facil, Southampton, Hants, England

关键词: Chronic rhinosinusitis;    chronic rhinosinusitis with nasal polyps;    nasal polyps;    intracellular bacteria;    Staphylococcus aureus;    mast cells;    bacterial superantigens;    staphylococcal enterotoxin B;   

DOI  :  10.1016/j.jaci.2019.06.013

来源: Elsevier

【 摘 要 】

Background: Chronic rhinosinusitis (CRS) with nasal polyps is a common chronic condition. The exact cause of nasal polyps remains unknown. Recently, we made the novel observation of intracellular localization of Staphylococcus aureus within mast cells in nasal polyps. Objective: This follow-up study aimed to further characterize interactions between S aureus and mast cells in this setting and elucidate potential internalization mechanisms with particular emphasis on the role of staphylococcal enterotoxin B (SEB). Methods: A prospective study was performed using an explant tissue model with ex vivo inferior turbinate mucosa obtained from patients with chronic rhinosinusitis with nasal polyps (n = 7) and patients without CRS (n = 5). Immunohistochemistry was used to characterize S aureus uptake into mast cells and investigate the effects of SEB on this process. An in vitro cell-culture model was used to investigate mast cell-S aureus interactions by using a combination of fluorescent in situ hybridization, confocal laser scanning microscopy, scanning electron microscopy, transmission electron microscopy, and proliferation assays. Results: S aureus was captured by extracellular traps and entered mast cells through phagocytosis. Proliferating intracellular S aureus led to the expansion and eventual rupture of mast cells, resulting in release of viable S aureus into the extracellular space. The presence of SEB appeared to promote internalization of S aureus into mast cells. Conclusion: This study provides new insights into the interactions between S aureus and mast cells, including the internalization process, and demonstrates a prominent role for SEB in promoting uptake of the bacteria into these cells.

【 授权许可】

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