Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:140

Article

Salimi, Maryam¹  Stoger, Linda^2,3  Liu, Wei²  Go, Simei²  Pavord, Ian²  Klenerman, Paul⁴  Ogg, Graham¹  Xue, Luzheng^2,3 

[1] Univ Oxford, John Radcliffe Hosp, NIHR Biomed Res Ctr, Weatherall Inst Mol Med,MRC Human Immunol Unit, Oxford, England

[2] Univ Oxford, Nuffield Dept Med, Resp Med Unit, Oxford, England

[3] Univ Oxford, Core Translat Immunol Lab, Oxford NIHR Biomed Res Ctr, Oxford, England

[4] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Med, Translat Gastroenterol Unit, Oxford, England

关键词: Group 2 innate lymphoid cell;    leukotriene E-4;    prostaglandin D2;    IL-25;    IL-33;    IL-2;    thymic stromal lymphopoietin;    atopic dermatitis;   

DOI  :  10.1016/j.jaci.2016.12.958

来源: Elsevier

【 摘 要 】

Background: Group 2 innate lymphoid cells (ILC2s) are a potential innate source of type 2 cytokines in the pathogenesis of allergic conditions. Epithelial cytokines (IL-33, IL-25, and thymic stromal lymphopoietin [TSLP]) and mast cell mediators (prostaglandin D-2 [PGD(2)]) are critical activators of ILC2s. Cysteinyl leukotrienes (cysLTs), including leukotriene (LT) C-4, LTD4, and LTE4, are metabolites of arachidonic acid and mediate inflammatory responses. Their role in human ILC2s is still poorly understood. Objectives: We sought to determine the role of cysLTs and their relationship with other ILC2 stimulators in the activation of human ILC2s. Methods: For ex vivo studies, fresh blood from patients with atopic dermatitis and healthy control subjects was analyzed with flow cytometry. For in vitro studies, ILC2s were isolated and cultured. The effects of cysLTs, PGD(2), IL-33, IL-25, TSLP, and IL-2 alone or in combination on ILC2s were defined by using chemotaxis, apoptosis, ELISA, Luminex, quantitative RT-PCR, and flow cytometric assays. The effect of endogenous cysLTs was assessed by using human mast cell supernatants. Results: Human ILC2s expressed the LT receptor CysLT1, levels of which were increased in atopic subjects. CysLTs, particularly LTE4, induced migration, reduced apoptosis, and promoted cytokine production in human ILC2s in vitro. LTE4 enhanced the effect of PGD(2), IL-25, IL-33, and TSLP, resulting in increased production of type 2 and other proinflammatory cytokines. The effect of LTE4 was inhibited by montelukast, a CysLT(1) antagonist. Interestingly, addition of IL-2 to LTE4 and epithelial cytokines significantly amplified ILC2 activation and upregulated expression of the receptors for IL-33 and IL-25. Conclusion: CysLTs, particularly LTE4, are important contributors to the triggering of human ILC2s in inflammatory responses, particularly when combined with other ILC2 activators.

【 授权许可】

Free