Identification of TNF-related apoptosis-inducing ligand and other molecules that distinguish inflammatory from resident dendritic cells in patients with psoriasis

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:125

Article

Zaba, Lisa C.¹  Fuentes-Duculan, Judilyn¹  Eungdamrong, Narat John¹  Johnson-Huang, Leanne M.¹  Nograles, Kristine E.¹  White, Traci R.¹  Pierson, Katherine C.¹  Lentini, Tim¹  Suarez-Farinas, Mayte^1,2  Lowes, Michelle A.¹  Krueger, James G.¹ 

[1] Rockefeller Univ, Lab Investigat Dermatol, New York, NY 10065 USA

[2] Rockefeller Univ, Ctr Clin & Translat Sci, New York, NY 10065 USA

关键词: TRAIL;    psoriasis;    dendritic cell;    inflammation;    CCL20;    Toll-like receptor 1;    Toll-like receptor 2;    S100A12;    CD32;   

DOI  :  10.1016/j.jaci.2010.03.018

来源: Elsevier

【 摘 要 】

Background: Previous work has identified CD11c(+)CD1c(-) dendritic cells (DCs) as the major inflammatory dermal DC population in patients with psoriasis vulgaris and CD1c(+) DCs as the resident cutaneous DC population. Objective: We sought to further define molecular differences between these 2 myeloid dermal DC populations. Methods: Inflammatory and resident DCs were single-cell sorted from lesional skin biopsy specimens of patients with psoriasis, and the transcriptome of CD11c(+)CD1c(-) versus CD1c(+) DCs was determined. Results were confirmed with RT-PCR, flow cytometry, immunohistochemistry, and double-labeled immunofluorescence. Human keratinocytes were cultured for functional studies. Results: TNF-related apoptosis-inducing ligand (TRAIL), Toll-like receptors 1 and 2, S1000A12/ENRAGE, CD32, and many other inflammatory products were differentially expressed in inflammatory DCs compared with resident DCs. Flow cytometry and immunofluorescence confirmed higher protein expression on CD1c(-) versus CD1c(+) DCs. TRAIL receptors, death receptor 4, and decoy receptor 2 were expressed in keratinocytes and dermal cells. In vitro culture of keratinocytes with TRAIL induced CCL20 chemokine. Conclusions: CD11c(+)CD1c(-) inflammatory DCs in psoriatic lesional skin express a wide range of inflammatory molecules compared with skin-resident CD1c(+) DCs. Some molecules made by inflammatory DCs, including TRAIL, could have direct effects on keratinocytes or other skin cell types to promote disease pathogenesis. (J Allergy Clin Immunol 2010;125:1261-8.)

【 授权许可】

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