Characterization of Phl p 4, a major timothy grass (Phleum pratense) pollen allergen

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:98

Article

Fischer, S ; Grote, M ; Fahlbusch, B ; Muller, WD ; Kraft, D ; Valenta, R

关键词: grass pollen allergy;    Phl p 4;    trypsin resistance;    monoclonal antibodies;    cross-reactivity;    Amb a 1;    ragweed allergy;    pollen-specific expression;   

DOI  :  10.1016/S0091-6749(96)70242-7

来源: Elsevier

【 摘 要 】

Background: Group 4 grass pollen allergens represent glycoproteins with a molecular weight of 50 to 60 kd, which are present in many grass species. Almost 75% of patients allergic to grass pollen display IgE reactivity to group 4 allergens, which hence can be regarded as major grass pollen allergens. Objective: In this study attempts were made to obtain information regrading the immunologic properties, localization, and occur ence of Phl p 4 and related allergens. Methods: Phl p 4 was detected in timothy grass pollen extracts by immunoblotting with serum IgE and monoclonal antibodies and was localized in pollen by immunelectron microscopy. A peptide sequence from Phl p 4 was obtained by amino acid sequencing. The resistance of Phl p 4 against trypsin was analyzed after treatment of timothy grass pollen extracts with serum IgE and monoclonal antibodies. Cross-reactivities between Phl p 4 and Amb a 1, the major allergen of ragweed, were studied by using monoclonal antibodies and by IgE-inhibition studies. Results: Phl p 4 was characterized as a trypsin-resistant major timothy grass pollen allergen. By immunelectron microscopy Phl p 4 was localized in the exine, cytoplasm, and amyloplast of timothy grass pollen. Significant sequence similarities of a Phl p 4 10 amino acid peptide with Amb a 1, the major ragweed allergen, could be Sound. The immunologic similarity of Phl p 4 and Amb a 1 was confirmed by cross-reactivity of monoclonal antibodies and patients' IgE. Conclusion: Phl p 4 represents a trypsin-resistant major. timothy grass pollen allergen with immunologic similarities to the major ragweed allergen Amb a 1 and therefore must be considered an important cross-reactive component in grass pollen and weed pollen allergy.

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