Bronchial mucosal IFN-α/β and pattern recognition receptor expression in patients with experimental rhinovirus-induced asthma exacerbations

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:143

Article

Zhu, Jie^1,2  Message, Simon D.^1,2,3  Mallia, Patrick^1,2,3  Kebadze, Tatiana^1,2  Contoli, Marco^1,2,3,4,5  Ward, Christine K.⁶  Barnathan, Elliot S.⁶  Mascelli, Mary Ann⁶  Kon, Onn M.³  Papi, Alberto^4,5  Stanciu, Luminita A.^1,2  Edwards, Michael R.^1,2  Jeffery, Peter K.^1,2  Johnston, Sebastian L.^1,2,3 

[1] Imperial Coll London, Natl Heart & Lung Inst, Med Res Council, Airway Dis Infect, London, England

[2] Imperial Coll London, Asthma UK Ctr Allerg Mech Asthma, London, England

[3] Imperial Coll Healthcare NHS Trust, London, England

[4] Univ Ferrara, Res Ctr Asthma, Ferrara, Italy

[5] Univ Ferrara, COPD, Ferrara, Italy

[6] Centocor Inc, Malvern, PA USA

关键词: Asthma exacerbation;    rhinovirus infection;    type I interferon;    pattern recognition receptors;   

DOI  :  10.1016/j.jaci.2018.04.003

来源: Elsevier

【 摘 要 】

Background: The innate immune system senses viral infection through pattern recognition receptors (PRRs), leading to type I interferon production. The role of type I interferon and PPRs in rhinovirus-induced asthma exacerbations in vivo are uncertain. Objectives: We sought to compare bronchial mucosal type I interferon and PRR expression at baseline and after rhinovirus infection in atopic asthmatic patients and control subjects. Methods: Immunohistochemistry was used to detect expression of IFN-alpha, IFN-beta, and the PRRs: Toll-like receptor 3, melanoma differentiation-associated gene 5, and retinoic acid-inducible protein I in bronchial biopsy specimens from 10 atopic asthmatic patients and 15 nonasthmatic nonatopic control subjects at baseline and on day 4 and 6 weeks after rhinovirus infection. Results: We observed IFN-alpha/beta deficiency in the bronchial epithelium at 3 time points in asthmatic patients in vivo. Lower epithelial IFN-alpha/beta expression was related to greater viral load, worse airway symptoms, airway hyperresponsiveness, and reductions in lung function during rhinovirus infection. We found lower frequencies of bronchial subepithelial monocytes/macrophages expressing IFN-alpha/beta in asthmatic patients during infection. Interferon deficiency at baseline was not accompanied by deficient PRR expression in asthmatic patients. Both epithelial and subepithelial PRR expression were induced during rhinovirus infection. Rhinovirus infection-increased numbers of subepithelial interferon/PRR-expressing inflammatory cells were related to greater viral load, airway hyperresponsiveness, and reductions in lung function. Conclusions: Bronchial epithelial IFN-alpha/beta expression and numbers of subepithelial IFN-alpha/beta-expressing monocytes/macrophages during infection were both deficient in asthmatic patients. Lower epithelial IFN-alpha/beta expression was associated with adverse clinical outcomes after rhinovirus infection in vivo. Increases in numbers of subepithelial cells expressing interferon/PRRs during infection were also related to greater viral load/illness severity.

【 授权许可】

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