Kinetics of IL-10 production after segmental antigen challenge of atopic asthmatic subjects

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:106

Article

Colavita, AM ; Hastie, AT ; Musani, AI ; Pascual, RM ; Reinach, AJ ; Lustine, HT ; Galati, SA ; Zangrilli, JG ; Fish, JE ; Peters, SP

关键词: interleukin-10;    allergic asthma;    cytokines;    macrophages;    eosinophils;    glucocorticoids;    segmental antigen challenge;    bronchoalveolar lavage;    immunocytochemistry;   

DOI  :  10.1067/mai.2000.110475

来源: Elsevier

【 摘 要 】

Background: IL-10 is an anti-inflammatory cytokine made by lymphocytes, monocytes-macrophages, and eosinophils, and it may have an important role in regulating the asthmatic inflammatory response. IL-10 levels have been reported to be reduced in asthmatic airways, potentially contributing to more intense inflammation. Objective: We sought to determine whether IL-10 levels were deficient in patients with mild asthma compared with controls and to determine whether IL-10 levels were associated with the resolution of eosinophilic inflammation. Methods: We quantified IL-10 levels in the bronchoalveolar lavage (BAL) fluid (ELISA), BAL cells (quantitative immuno-cytochemistry), purified alveolar macrophages-monocytes studied ex vivo (ELISA), before (day 1) and after (24 hours [day 2], 1 week [day 9], and 2 weeks [day 16]) segmental antigen challenge (SAC), and investigated the effect of glucocorticoid treatment on ex vivo macrophage-monocyte IL-10 production. Results: IL-10 levels were significantly higher in the BAL fluid of mild asthmatic subjects who demonstrated a dual reaction (both early and late) after whole lung ragweed inhalation challenge compared with nonallergic, nonasthmatic control subjects before and 24 hours and 1 week after SAG. Macrophages-monocytes obtained before and after SAC from asthmatic patients also secreted increased amounts of IL-10 ex vivo than those from 0controls. Dexamethasone did not significantly change spontaneous IL-10 secretion from macrophages-monocytes in vitro. Quantitative immunocytochemical analysis of BAL cells demonstrated increased IL-10 in macrophages 24 hours after SAC and a similar trend in eosinophils. Conclusion: IL-10 is not deficient in mild asthma. Furthermore, BAL IL-20 levels are significantly higher in asthmatic subjects with a dual response than in control subjects before and after SAG. The increase in IL-10 was coincident with the initial increase in BAL eosinophils, although BAL eosinophilia persisted after IL-10 levels had returned to baseline, suggesting that the increased IL-10 levels could not promptly terminate this localized eosinophilic response.

【 授权许可】

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