Expression of the IL-4 receptor α-subunit is increased in bronchial biopsy specimens from atopic and nonatopic asthmatic subjects

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:102

Article

Kotsimbos, TC ; Ghaffar, O ; Minshall, EM ; Humbert, M ; Durham, SR ; Pfister, R ; Menz, G ; Kay, AB ; Hamid, QA

关键词: atopic asthma;    intrinsic asthma;    IL-4 receptor;    IL-4;    IgE;   

DOI  :  10.1016/S0091-6749(98)70029-6

来源: Elsevier

【 摘 要 】

Background: Recent studies have provided evidence for increased IL-4 expression in the airways of atopic and nonatopic asthmatic subjects. IL-4 is believed to perform important regulatory roles in asthma: however, the expression of the IL-4 receptor has not been investigated. In this study we examined the mRNA and protein expression of the specific alpha-subunit of the IL-4 receptor (alpha IL-1R) in bronchial biopsy specimens obtained from atopic and nonatopic asthmatic subjects. Methods: Asthmatic subjects and nonasthmatic control subjects were recruited, and lung function measurements were performed before bronchoscopy, Endobronchial biopsy specimens were examined for the presence of alpha IL-1R mRNA and immunoreactivity by using in situ hybridization and immunocytochemistry, respectively. Results: alpha IL-4R mRNA-positive and immunoreactive cells were detected in the epithelium and subepithelium in biopsy specimens from all subjects. Expression of alpha IL-4R mRNA and protein was significantly increased in the epithelium and subepithelium of biopsy; specimens from atopic asthmatic subjects compared with atopic control subjects (P < .05 and r < .001, respectively). Epithelial alpha IL-4R mRNA expression and immunoreactivity did not differ significantly between nonatopic asthmatic subjects and nonatopic control subjects. Although the numbers of alpha IL-4R mRNA-positive cells were augmented in the submucosa of intrinsic asthmatic subjects compared with nonatopic control subjects (P < .05), alpha IL-4R immunoreactivity did not differ significantly between these groups. Increased alpha IL-4R immunoreactive signals were also detected in the endothelial cell layer in both atopic and intrinsic asthmatic subjects compared with atopic and nonatopic control subjects, respectively (P < .05). Combined in situ hybridization immunocytochemistry performed on biopsy sections from asthmatic and control subjects demonstrated alpha IL-4R mRNA expression in CD3-positive T cells and tryptase-positive e mast cells, with T cells comprising the larger proportion of alpha IL-4R mRNA-positive cells. Numbers of alpha IL-4R mRNA-positive or immunoreactive cells did not correlate with CD3-positive cell numbers, numbers of IL-4 mRNA-positive cells, or indices of pulmonary function. Conclusion: These results demonstrate constitutive alpha IL-4R expression in normal airways and enhanced expression in airway tissue from asthmatic individuals.

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