Enhanced innate type 2 immune response in peripheral blood from patients with asthma

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:134

Article

Bartemes, Kathleen R.^1,2  Kephart, Gail M.¹  Fox, Stephanie J.¹  Kita, Hirohito^1,2 

[1] Mayo Clin, Dept Internal Med, Div Allerg Dis, Rochester, MN 55905 USA

[2] Mayo Clin, Dept Immunol, Rochester, MN 55905 USA

关键词: IL-33;    IL-25;    IL-5;    IL-13;    ILC2;    innate immunity;    asthma;    allergy;   

DOI  :  10.1016/j.jaci.2014.06.024

来源: Elsevier

【 摘 要 】

Background: In mice, group 2 innate lymphoid cells (ILC2s) likely mediate helminth immunity, inflammation, and tissue repair and remodeling. However, the involvement of ILC2s in human diseases, such as asthma, is not well understood. Objectives: The goals of this study were to investigate whether peripheral blood specimens can be used to monitor innate type 2 immunity in human subjects and to examine whether ILC2s are involved in human asthma. Methods: PBMCs from subjects with allergic asthma (AA), subjects with allergic rhinitis (AR), or healthy control (HC) subjects were cultured in vitro with IL-25 or IL-33. Flow cytometry and cell sorting were used to identify, isolate, and quantitate ILC2s in PBMCs. Results: Human PBMCs produced IL-5 and IL-13 when stimulated with IL-33 or IL-25 in the presence of IL-2 without antigens. In addition, IL-7 or thymic stromal lymphopoietin were able to replace IL-2. The cell population with phenotypic ILC2 characteristics, lineage(-) CD127(+) CRTH2(+) cells, responded to IL-33 and produced large quantities of IL-5 and IL-13 but undetectable levels of IL-4. PBMCs from subjects with AA produced significantly larger amounts of IL-5 and IL-13 in response to IL-25 or IL-33 than from subjects with AR or HC. The prevalence of ILC2s in blood was greater in the AA group than in the AR group or the HC group. Conclusions: Innate type 2 immune responses are increased in asthma but not in AR, suggesting potential differences in the immunopathogenesis of these diseases. Peripheral blood is useful for evaluating innate type 2 immunity in humans.

【 授权许可】

Free