Coexpression of type 2 immune targets in sputum-derived epithelial and dendritic cells from asthmatic subjects

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:136

Article

Bleck, Bertram¹  Kazeros, Angeliki¹  Bakal, Keren¹  Garcia-Medina, Lymaris¹  Adams, Alexandra¹  Liu, Mengling^2,3  Lee, Richard A.¹  Tse, Doris B.¹  Chiu, Amanda¹  Grunig, Gabriele²  Egan, John P., III¹  Reibman, Joan^1,2 

[1] NYU, Langone Med Ctr, Dept Med, New York, NY 10016 USA

[2] NYU, Langone Med Ctr, Dept Environm Med, New York, NY 10016 USA

[3] NYU, Sch Med, Dept Populat Hlth, New York, NY 10016 USA

关键词: Asthma;    sputum;    bronchial epithelial cells;    dendritic cells;    thymic stromal lymphopoietin;    IL-33;    OX40 ligand;    CCL17;   

DOI  :  10.1016/j.jaci.2014.12.1950

来源: Elsevier

【 摘 要 】

Background: Noninvasive sputum sampling has enabled the identification of biomarkers in asthmatic patients. Studies of discrete cell populations in sputum can enhance measurements compared with whole sputum in which changes in rare cells and cell-cell interactions can be masked. Objective: We sought to enrich for sputum-derived human bronchial epithelial cells (sHBECs) and sputum-derived myeloid type 1 dendritic cells (sDCs) to describe transcriptional coexpression of targets associated with a type 2 immune response. Methods: A case-control study was conducted with patients with mild asthma (asthmatic cases) and healthy control subjects. Induced sputum was obtained for simultaneous enrichment of sHBECs and sDCs by using flow cytometry. Quantitative PCR was used to measure mRNA for sHBEC thymic stromal lymphopoietin (TSLP), IL33, POSTN, and IL25 and downstream targets in sDCs (OX40 ligand [OX40L], CCL17, PPP1R14A, CD1E, CD1b, CD80, and CD86). Results: Final analyses for the study sample were based on 11 control subjects and 13 asthmatic cases. Expression of TSLP, IL33, and POSTN mRNA was increased in sHBECs in asthmatic cases (P = .001, P = .05, and P = .04, respectively). Expression of sDC OX40L and CCL17 mRNA was increased in asthmatic cases (P = .003 and P = .0001, respectively). sHBEC TSLP mRNA expression was strongly associated with sDC OX40L mRNA expression (R = 0.65, P = .001) and less strongly with sDC CCL17 mRNA expression. sHBEC IL33 mRNA expression was associated with sDC OX40L mRNA expression (R = 0.42, P = .04) but not sDC CCL17 mRNA expression. Conclusions: Noninvasive sampling and enrichment of select cell populations from sputum can further our understanding of cell-cell interactions in asthmatic patients with the potential to enhance endotyping of asthmatic patients.

【 授权许可】

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