Skin microbiome before development of atopic dermatitis: Early colonization with commensal staphylococci at 2 months is associated with a lower risk of atopic dermatitis at 1 year

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:139

Article

Kennedy, Elizabeth A.¹  Connolly, Jennifer²  Hourihane, Jonathan O'B.²  Fallon, Padraic G.^3,4  McLean, W. H. Irwin⁷  Murray, Deirdre²  Jo, Jay-Hyun¹  Segre, Julia A.⁸  Kong, Heidi H.¹  Irvine, Alan D.^3,4,5,6 

[1] NCI, Dermatol Branch, Ctr Canc Res, NIH, Bldg 10, Bethesda, MD 20892 USA

[2] Univ Coll, Paediat & Child Hlth, Cork, Ireland

[3] Cork Univ Matern Hosp, Irish Ctr Fetal & Neonatal Translat INFANT Res, Cork, Ireland

[4] Trinity Coll Dublin, Med Clin, Dublin, Ireland

[5] Univ Dundee, Natl Childrens Res Ctr, Dundee DD1 4HN, Scotland

[6] Univ Dundee, Paediat Dermatol, Our Ladys Childrens Hosp Crumlin, Dundee DD1 4HN, Scotland

[7] Univ Dundee, Dermatol & Genet Med, Dundee DD1 4HN, Scotland

[8] NHGRI, Translat & Funct Genom Branch, NIH, Bethesda, MD 20892 USA

关键词: Staphylococcus aureus;    atopic dermatitis;    skin;    microbiome;    longitudinal birth cohort;    16S sequencing;   

DOI  :  10.1016/j.jaci.2016.07.029

来源: Elsevier

【 摘 要 】

Background: Disease flares of established atopic dermatitis (AD) are generally associated with a low-diversity skin microbiota and Staphylococcus aureus dominance. The temporal transition of the skin microbiome between early infancy and the dysbiosis of established AD is unknown. Methods: We randomly selected 50 children from the Cork Babies After SCOPE: Evaluating the Longitudinal Impact Using Neurological andNutritional Endpoints (BASELINE) longitudinal birth cohort for microbiome sampling at 3 points in the first 6 months of life at 4 skin sites relevant to AD: the antecubital and popliteal fossae, nasal tip, and cheek. We identified 10 infants with AD and compared them with 10 randomly selected control infants with no AD. We performed bacterial 16S ribosomal RNA sequencing and analysis directly from clinical samples. Results: Bacterial community structures and diversity shifted over time, suggesting that age strongly affects the skin microbiome in infants. Unlike established AD, these patients with infantile AD did not have noticeably dysbiotic communities before or with disease and were not colonized by S aureus. In comparing patients and control subjects, infants who had affected skin at month 12 had statistically significant differences in bacterial communities on the antecubital fossa at month 2 compared with infants who were unaffected at month 12. In particular, commensal staphylococci were significantly less abundant in infants affected at month 12, suggesting that this genus might protect against the later development of AD. Conclusions: This study suggests that 12-month-old infants with AD were not colonized with S aureus before having AD. Additional studies are needed to confirm whether colonization with commensal staphylococci modulates skin immunity and attenuates development of AD.

【 授权许可】

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