Cathelicidin deficiency predisposes to eczema herpeticum

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:117

Article

Howell, MD ; Wollenberg, A ; Gallo, RL ; Flaig, M ; Streib, JE ; Wong, C ; Pavicic, T ; Boguniewicz, M ; Leung, DYM

关键词: antimicrobial peptides;    herpes simplex virus;    atopic dermatitis;    eczema herpeticum;   

DOI  :  10.1016/j.jaci.2005.12.1345

来源: Elsevier

【 摘 要 】

Background: The cathelicidin family of antimicrobial peptides is an integral component of the innate immune response that exhibits activity against bacterial, fungal, and viral pathogens. Eczema herpeticum (ADEH) develops in a subset of patients with atopic dermatitis (AD) because of disseminated infection with herpes simplex virus (HSV). Objective: This study investigated the potential role of cathelicidins in host susceptibility to HSV infection. Methods: Glycoprotein D was measured by means of real-time RT-PCR as a marker of HSV replication in skin biopsy specimens and human keratinocyte cultures. Cathelicidin expression was evaluated in skin biopsy specimens from patients with AD (n = 10) without a history of HSV skin infection and from patients with ADEH (n = 10). Results: The cathelicidin peptide LL-37 (human cathelicidin) exhibited activity against HSV in an antiviral assay, with significant killing (P <.001) within the physiologic range. The importance of cathelicidins in antiviral skin host defense was confirmed by the observation of higher levels of HSV-2 replication in cathelicidin-deficient (Cnlp(-/-)) mouse skin (2.6 +/- 0.5 pg HSV/pg GAPDH, P <.05) compared with that seen in skin from their wild-type counterparts (0.9 +/- 0.3). Skin from patients with ADEH exhibited significantly (P <.05) lower levels of cathelicidin protein expression than skin from patients with AD. We also found a significant inverse correlation between cathelicidin expression and serum IgE levels (r(2) = 0.46, P <.05) in patients with AD and patients with ADEH. Conclusion: This study demonstrates that the cathelicidin peptide LL-37 possesses antiviral activity against HSV and demonstrates the importance of variable skin expression of cathelicidins in controlling susceptibility to ADEH. Additionally, serum IgE levels might be a surrogate marker for innate immune function and serve as a biomarker for which patients with AD are susceptible to ADEH. Clinical implications: A deficiency of LL-37 might render patients with AD susceptible to ADEH. Therefore increasing production of skin LL-37 might prevent herpes infection in patients with AD.

【 授权许可】

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