Recognition of pollen-derived phosphatidylethanolamine by human CD1d-restricted γδ T cells

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:117

Article

Russano, Anna M. ; Agea, Elisabetta ; Corazzi, Lanfranco ; Postle, Antyony D. ; De Libero, Gennaro ; Porcelli, Steven ; de Benedictis, Fernando M. ; Spinozzi, Fabrizio

关键词: cypress pollen;    olive pollen;    phospholpids;    phosphatidyl-ethanolamine;    rhinitis;    nasal mucosa;    gamma delta T lymphocytes;    T-cell clones;    CD1-restriction;    cytokines;    IL-4;    skin prick tests;    spicific IgE;   

DOI  :  10.1016/j.jaci.2006.01.001

来源: Elsevier

【 摘 要 】

Background: Evidences from mice and human beings indicate that gamma delta T cells could be relevant in recognition of stress-induced self and/or yet unidentified inhaled foreign antigens. Their specificity differs from classic MHC-restricted alpha beta T cells and involves the immunoglobulin-like structure of they gamma delta T-cell receptor with the recognition of small organic molecules, alkylamines, and self lipid compounds presented by CD1(+) dendritic cells. Objective: Because CD1 receptors are mainly devoted to lipid antigen presentation, we sought to determine whether exogenous pollen membrane lipids may act as allergens for CD1-restricted gamma delta T cells. Methods: Peripheral blood and nasal mucosa-associated gamma delta T cells were cloned from normal controls and cypress-sensitive subjects and tested for their antigen specificity and CD1-restriction with phospholipids extracted from tree pollen grains, as well with other natural or synthetic compounds. Phospholipid reactivity of cloned gamma delta T cells was measured by mean of proliferative response and cytokine release as well as by testing their helper activity on IgE production in vitro and in vivo. Results: Cloned gamma delta T lymphocytes from subjects with allergy, but not normal controls, were found to recognize pollen-derived phosphatidyl-ethanolamine (PE) in a CD1d-restricted fashion. Only 16:0/18:2 and 18:2/18:2 PE were stimulatory, whereas no response was recorded for disaturated PE, phosphatidylcholine, neutral lipids, or protein extract. Proliferating clones secreted both T(H)1-type and T(H)2-type cytokines and drove IgE production in vitro and in vivo. Conclusion: CD1d-restricted gamma delta T cells specific for phospholipids can represent a key mucosal regulatory subset for the control of early host reactivity against tree pollens. Clinical implications: By knowing how lipid allergen constituents interact with mucosal immune system, we can expand our possibilities in diagnostic and therapeutic interventions.

【 授权许可】

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