Principles, potential, and limitations of ex vivo basophil activation by flow cytometry in allergology: A narrative review

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:147

Review

Ebo, Didier G.^1,2,3,4  Bridts, Chris H.^1,2,3  Mertens, Christel H.^1,2,3  Sabato, Vito^1,2,3,4 

[1] Antwerp Univ, Fac Med & Hlth Sci, Dept Immunol, Allergol,Rheumatol, Antwerp, Belgium

[2] Antwerp Univ, Infla Med Ctr Excellence, Antwerp, Belgium

[3] Antwerp Univ Hosp, Immunol, Allergol, Rheumatol, Antwerp, Belgium

[4] AZ Jan Palfijn Gent, Dept Immunol & Allergol, Ghent, Belgium

关键词: Allergy;    anaphylaxis;    basophil;    basophil activation;    CD63;    CD203c;    flow cytometry;    histamine;    HistaFlow;    IgE;    mast cell;    mast cell activation;   

DOI  :  10.1016/j.jaci.2020.10.027

来源: Elsevier

【 摘 要 】

The major challenge of allergy diagnosis lies in the development of accessible and reliable diagnostics allowing correct prediction of the clinical outcome following exposure to the offending allergen(s) and cross-reactive structures. Since the late nineties, evidence has accumulated that flow-assisted analysis and quantification of ex vivo?activated basophils (according to the basophil activation test [BAT]) might meet this requirement for different IgE-dependent allergies and particular forms of autoimmune urticaria. Other so-called nondiagnostic applications of the BAT involve therapeutic monitoring, followup of natural histories, and identification of allergenic recognition sites. However, it has also become clear that appropriate use of the BAT necessitates knowledge about degranulation metrics and guidance to guarantee correct execution and interpretation of the results. Here, we have reviewed the most relevant applications and limitations of the BAT. Some personal statements and views about its perspectives are made. (J Allergy Clin Immunol 2021;147:1143-53.)

【 授权许可】

Free