Tear and conjunctival changes during the allergen-induced early- and late-phase responses

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:106

Article

Bacon, AS ; Ahluwalia, P ; Irani, AM ; Schwartz, LB ; Holgate, ST ; Church, MK ; McGill, JI

关键词: allergy;    conjunctivitis;    histamine;    tryptase;    eosinophil cationic protein;    mast cell;    basophil;    eosinophil;    T cell;    E-selectin;    intercellular adhesion molecule 1;    vascular adhesion molecule 1;   

DOI  :  10.1067/mai.2000.110930

来源: Elsevier

【 摘 要 】

Background: Allergic eye disease is common, but little is known about the underlying disease mechanisms. Conjunctival allergen challenge causes symptoms similar to those of seasonal allergic conjunctivitis and is a useful model to study. Objective: We have used allergen challenge to investigate the course of the ocular response, tear inflammatory mediators, tissue adhesion protein expression, and cellular infiltration. Methods: Eighteen atopic patients and 4 nonatopic control subjects were challenged with extracted mixed grass or Dermatophagoides pteronyssinus in one eye and control vehicle in the other. The clinical response was recorded, and tears mere collected over a 6-hour period. Conjunctival biopsy specimens mere taken from the challenged eye at 6 or 24 hours. Results: An early-phase response (maximal at 20 minutes) showed a significant increase in tear histamine and tryptase levels, reducing to control levels again by 40 minutes. At 6 hours, a late-phase response occurred with increased symptoms, a second peak of tear histamine and eosinophil cationic protein but not tryptase, upregulation of the adhesion molecules E-selectin and intercellular adhesion molecule, and a cellular infiltrate of mast cells, neutrophils, eosinophils, macrophages, and basophils, with T cells increased only in bulbar biopsy specimens. Conclusions: The early peaks of tear histamine plus tryptase indicate that the mast cell is responsible for the early-phase response, but basophils may be involved in the late-phase response. Both tear and biopsy findings underline the significance of the late-phase response as the transition between a type I response and clinical disease.

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