Atrazine biodegradation by mycoinsecticide Metarhizium robertsii: Insights into its amino acids and lipids profile

JOURNAL OF ENVIRONMENTAL MANAGEMENT

卷:262

Article

Szewczyk, Rafal¹  Rozalska, Sylwia²  Mironenka, Julia²  Bernat, Przemyslaw² 

[1] Ctr Clin & Aesthet Med DiMed, Legionow 40-19, PL-90702 Lodz, Poland

[2] Univ Lodz, Fac Biol & Environm Protect, Inst Microbiol Biotechnol & Immunol, Dept Ind Microbiol & Biotechnol, Banacha 12-16, PL-90237 Lodz, Poland

关键词: Atrazine;    Biodegradation;    Metarhizium;    Metabolomics;    Lipidomics;    Bioinsecticide;   

DOI  :  10.1016/j.jenvman.2020.110304

来源: Elsevier

【 摘 要 】

Atrazine, is one of major concern pesticides contaminating agricultural areas and ground water. Its microbial biodegradation seems to be the most efficient in terms of economic and environmental benefits. In the present work the cometabolic biodegradation of atrazine by the fungus Metarhizum robertsii IM 6519 during 10-day batch cultures was characterized. The herbicide was transformed to several hydroxy-, dechlorinated or dealkylated metabolites with the involvement of cytochrome P450 monooxygenases. The obtained metabolomics data revealed that atrazine induced oxidative stress (increased the levels of L-proline, L-ornithine, L-arginine, GABA and L-methionine), disruptions of the carbon and nitrogen metabolism (L-aspartic acid, L-asparagine, L-tyrosine, L-threonine, L-isoleucine, L-phenylalanine, 1-methyl-L-histidine, L-tryptophan, L-valine, L-alanine, O-phospho-Lserine, L-sarcosine or L-lysine) and caused an increase in the membrane fluidity (a rise in the phosphatidylcholines/phosphatidylethanolamines (PC/PE) ratio together with the growth of the taurine level). The increased level of hydroxyl derivatives of linoleic acid (9-HODE and 13-HODE) confirmed that atrazine induced lipid peroxidation. The presented results suggesting that M. robertsii IM 6519 might be applied in atrazine biodegradation and may bring up the understanding of the process of triazine biodegradation by Metarhizum strains.

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