Electron flow of biological H2 production by sludge under simple thermal treatment: Kinetic study

JOURNAL OF ENVIRONMENTAL MANAGEMENT

卷:250

Article

Amin, Mohammad Mehdi^1,2  Taheri, Ensiyeh^1,2,3  Bina, Bijan^1,2  van Ginkel, Steven W.⁴  Ghasemian, Mohammad¹  Puad, Noor Illi Mohamad⁵  Fatehizadeh, Ali^1,2 

[1] Isfahan Univ Med Sci, Environm Res Ctr, Res Inst Primordial Prevent Noncommunicable Dis, Esfahan, Iran

[2] Isfahan Univ Med Sci, Sch Hlth, Dept Environm Hlth Engn, Esfahan, Iran

[3] Isfahan Univ Med Sci, Sch Hlth, Student Res Comm, Esfahan, Iran

[4] Georgia Inst Technol, Sch Civil & Environm Engn, 200 Bobby Dodd Way, Atlanta, GA 30332 USA

[5] Int Islamic Univ Malaysia, Dept Biotechnol Engn Kulliyyah Engn, Bioproc & Mol Engn Res Unit BPMERU, Kuala Lumpur, Malaysia

关键词: Biohydrogen;    Electron flow model;    H-2 yield;    Kinetic model;    Stoichiometry;   

DOI  :  10.1016/j.jenvman.2019.109461

来源: Elsevier

【 摘 要 】

Mixed culture sludge has been widely used as a microbial consortium for biohydrogen production. Simple thermal treatment of sludge is usually required in order to eliminate any H-2-consuming bacteria that would reduce H-2 production. In this study, thermal treatment of sludge was carried out at various temperatures. Electron flow model was then applied in order to assess community structure in the sludge upon thermal treatment for biohydrogen production. Results show that the dominant electron sink was acetate (150-217 e(-) meq/mol glucose). The electron equivalent (e(-) eq) balances were within 0.8-18% for all experiments. Treatment at 100 degrees C attained the highest H-2 yield of 3.44 mol H-2/mol glucose from the stoichiometric reaction. As the treatment temperature increased from 80 to 100 degrees C, the computed acetyl-CoA and reduced form of ferredoxin (Fdred) concentrations increased from 13.01 to 17.34 e(-) eq (1.63-2.17 mol) and 1.34 to 4.18 e(-) eq (0.67-2.09 mol), respectively. The NADH(2) balance error varied from 3 to 10% and the term e(-)(Fd <-> NADH(2)) (m) in the NADH(2) balance was NADH(2) consumption (m = -1). The H-2 production was mainly via the Fd:hydrogenase system and this is supported with a good NADH(2) balance. Using the modified Gompertz model, the highest maximum H-2 production potential was 1194 mL whereas the maximum rate of H-2 production was 357 mL/h recorded at 100 degrees C of treatment.

【 授权许可】

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