Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:570

Article; Proceedings Paper

Montis, Costanza^1,2  Salvatore, Annalisa^1,2  Valle, Francesco^3,4  Paolini, Lucia^5,6  Carla, Francesco^7,8  Bergese, Paolo^5,6  Berti, Debora^1,2 

[1] Univ Florence, Dept Chem, Via Lastruccia 3, I-50019 Florence, Italy

[2] CSGI, Via Lastruccia 3, I-50019 Florence, Italy

[3] ISMN CNR, Via Gobetti 101, I-40129 Bologna, Italy

[4] CSGI, Via Gobetti 101, I-40129 Bologna, Italy

[5] Univ Brescia, Dept Mol & Translat Med, Viale Europa 11, I-25123 Brescia, Italy

[6] CSGI, Viale Europa 11, I-25123 Brescia, Italy

[7] European Synchrotron, ESRF, 71 Ave Martyrs, F-38000 Grenoble, France

[8] Diamond Light Source, Harwell Sci & Innovat Campus,Fermi Ave, Didcot OX11 0DE, Oxon, England

关键词: Extracellular vesicles;    Exosomes;    Supported lipid bilayers;    SPIONs;    Nano-bio interface;    Biomimetic membranes;   

DOI  :  10.1016/j.jcis.2020.03.014

来源: Elsevier

【 摘 要 】

Hypothesis: Extracellular Vesicles (EVs) are natural nanosized lipid vesicles involved in most intercellular communication pathways. Given their nature, they represent natural cell membrane models, with intermediate complexity between real and synthetic lipid membranes. Here we compare EVs-derived (EVSLB) and synthetic Supported Lipid Bilayers (SLBs) in the interaction with cationic superparamagnetic iron oxide nanoparticles (SPIONs). The aim is twofold: (i) exploit SPIONs as nanometric probes to investigate the features of EVSLBs as novel biogenic platforms; (ii) contribute at improving the knowledge on the behavior of SPIONs with biological interfaces. Experiments: Quartz Crystal Microbalance, X-ray Reflectivity, Grazing-incidence Small-angle X-ray Scattering, Atomic Force Microscopy, Confocal Microscopy data on SPIONs-EVSLB were systematically compared to those on SPIONs challenging synthetic SLBs, taken as references. Findings: The ensemble of experimental results highlights the much stronger interaction of SPIONs with EVSLBs with respect to synthetic SLBs. This evidence strongly supports the hypotheses on the peculiar structure of EVSLBs, with cushioned non-flat areas and extended exposed surface; in addition, it suggests that these features are relevant in the response of biogenic membranes to nano-objects. These findings contribute to the fundamental knowledge on EVSLBs, key for their development both as biomimetic membranes, or as platforms for biomedical applications. (C) 2020 Elsevier Inc. All rights reserved.

【 授权许可】

Free