Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:574

Article

Hall, Stephen C. L.^1,2,6  Clifton, Luke A.³  Tognoloni, Cecilia⁴  Morrison, Kerrie A.⁴  Knowles, Timothy J.¹  Kinane, Christian J.³  Dafforn, Tim R.¹  Edler, Karen J.⁴  Arnold, Thomas^2,3,4,5 

[1] Univ Birmingham, Sch Biosci, Edgbaston B15 2TT, England

[2] Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 ODE, Oxon, England

[3] Rutherford Appleton Lab, ISIS Neutron & Muon Source, Didcot OX11 0QX, Oxon, England

[4] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

[5] European Spallat Source ERIC, POB 176, SE-22100 Lund, Sweden

[6] Univ Warwick, Dept Chem, Gibbet Hill, Coventry CV4 7AL, W Midlands, England

关键词: Supported lipid bilayer;    Polymer-stabilized phospholipid nanodisc;    styrene-maleic acid lipid particle (SMALP);    styrene maleic acid (SMA);    Neutron reflectometry;    Lipid exchange;    Adsorption;   

DOI  :  10.1016/j.jcis.2020.04.013

来源: Elsevier

【 摘 要 】

Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phos-pholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins. (C) 2020 The Authors. Published by Elsevier Inc.

【 授权许可】

Free