On the control of dispersion interactions between biological membranes and protein coated biointerfaces

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:598

Article

Blackwell, Robert^1,2  Hemmerle, Arnaud^3,8  Baer, Andreas^1,2  Spaeth, Matthias^1,2  Peukert, Wolfgang⁴  Parsons, Drew^5,7  Sengupta, Kheya³  Smith, Ana-Suncana^1,2,6 

[1] Friedrich Alexander Univ Erlangen Nurnberg, IZNF, Dept Phys, PULS Grp, Cauerstr 3, D-91058 Erlangen, Germany

[2] Friedrich Alexander Univ Erlangen Nurnberg, IZNF, Interdisciplinary Ctr Nanostruct Films, Cauerstr 3, D-91058 Erlangen, Germany

[3] Aix Marseille Univ, Ctr Interdisciplinaire Nanosci Marseille, CNRS, UMR 7325, Campus Luminy, F-13288 Marseille 9, France

[4] Friedrich Alexander Univ Erlangen Nurnberg, Interdisciplinary Ctr Funct Particle Syst, Inst Particle Technol, Haberstr 9a, D-91058 Erlangen, Germany

[5] Univ Cagliari, Dept Chem & Geol Sci, I-09042 Monserrato, CA, Italy

[6] Rudjer Boskovic Inst, Div Phys Chem, Bijenicka Cesta 54, Zagreb 10000, Croatia

[7] Murdoch Univ, Discipline Phys Chem & Math, Coll Sci Hlth Engn & Edu, Murdoch, WA 6150, Australia

[8] SOLEIL, BP48, F-91192 Gif Sur Yvette, France

关键词: Biointerfaces;    Dispersion forces;    Lifshitz theory;    Van der Waals interactions;    Reflection interference contrast microscopy (RICM);   

DOI  :  10.1016/j.jcis.2021.02.078

来源: Elsevier

【 摘 要 】

Hypothesis: Interaction of cellular membranes with biointerfaces is of vital importance for a number of medical devices and implants. Adhesiveness of these surfaces and cells is often regulated by depositing a layer of bovine serum albumin (BSA) or other protein coatings. However, anomalously large separations between phospholipid membranes and the biointerfaces in various conditions and buffers have been observed, which could not be understood using available theoretical arguments. Methods: Using the Lifshitz theory, we here evaluate the distance-dependent Hamaker coefficient describing the dispersion interaction between a biointerface and a membrane to understand the relative positioning of two surfaces. Our theoretical modeling is supported by experiments where the biointerface is represented by a glass substrate with deposited BSA and protein layers. These biointerfaces are allowed to interact with giant unilamellar vesicles decorated with polyethylene glycol (PEG) using PEG lipids to & nbsp;mimic cellular membranes and their pericellular coat. Results: We demonstrate that careful treatment of the van der Waals interactions is critical for explaining the lack of adhesiveness of the membranes with protein-decorated biointerfaces. We show that BSA alone indeed passivates the glass, but depositing an additional protein layer on the surface BSA, or producing multiple layers of proteins and BSA results in repulsive dispersion forces responsible for 100 nm large equilibrium separations between the two surfaces. (C) 2021 Elsevier Inc. All rights reserved.

【 授权许可】

Free