Platelet-like particles improve fibrin network properties in a hemophilic model of provisional matrix structural defects

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:577

Article

Nandi, Seema^1,2,3  Sommerville, Laura⁴  Nellenbach, Kimberly^1,2,3  Mihalko, Emily^1,2,3  Erb, Mary^1,2  Freytes, Donald O.^1,2,3  Hoffman, Maureane⁴  Monroe, Dougald⁵  Brown, Ashley C.^1,2,3 

[1] Univ North Carolina Chapel Hill, Joint Dept Biomed Engn, Raleigh, NC USA

[2] North Carolina State Univ, Raleigh, NC 27607 USA

[3] North Carolina State Univ, Comparat Med Inst, Raleigh, NC 27607 USA

[4] Duke Univ, Dept Pathol, Durham, NC 27706 USA

[5] Univ N Carolina, Div Hematol Oncol, Chapel Hill, NC 27515 USA

关键词: Fibrin;    Biomimetic;    Platelet;    Hemophilia;    Biomaterials;   

DOI  :  10.1016/j.jcis.2020.05.088

来源: Elsevier

【 摘 要 】

Following injury, a fibrin-rich provisional matrix is formed to stem blood loss and provide a scaffold for infiltrating cells, which rebuild the damaged tissue. Defects in fibrin network formation contribute to impaired healing outcomes, as evidenced in hemophilia. Platelet-fibrin interactions greatly influence fibrin network structure via clot contraction, which increases fibrin density over time. Previously developed hemostatic platelet-like particles (PLPs) are capable of mimicking platelet functions including binding to fibrin fibers, augmenting clotting, and inducing clot retraction. In this study, we aimed to apply PLPs within a plasma-based in vitro hemophilia B model of deficient fibrin network structure to determine the ability of PLPs to improve fibrin structure and wound healing responses within hemophilia-like abnormal fibrin network formation. PLP impact on structurally deficient clot networks was assessed via confocal microscopy, a micropost deflection model, atomic force microscopy and an in vitro wound healing model of early cell migration within a provisional fibrin matrix. PLPs improved clot network density, force generation, and stiffness, and promoted fibroblast migration within an in vitro model of early wound healing under hemophilic conditions, indicating that PLPs could provide a biomimetic platform for improving wound healing events in disease conditions that cause deficient fibrin network formation. (C) 2020 Elsevier Inc. All rights reserved.

【 授权许可】

Free