Adsorption of T4 bacteriophages on planar indium tin oxide surface via controlled surface tailoring

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:468

Article

Liana, Ayu Ekajayanthi¹  Chia, Ed Win¹  Marquis, Christopher P.²  Gunawan, Cindy^1,3  Gooding, J. Justin^4,5  Amal, Rose¹ 

[1] Univ New S Wales, Sch Chem Engn, Sydney, NSW 2052, Australia

[2] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

[3] Univ Technol Sydney, Ithree Inst, Sydney, NSW 2007, Australia

[4] Univ New S Wales, Sch Chem, Sydney, NSW 2052, Australia

[5] Univ New S Wales, Australian Ctr NanoMed, Sydney, NSW 2052, Australia

关键词: Bacteriophage;    Adsorption;    Virus-surface interactions;    Bio-nano interactions;    Surface modifications;   

DOI  :  10.1016/j.jcis.2016.01.052

来源: Elsevier

【 摘 要 】

The work investigates the influence of surface physicochemical properties of planar indium tin oxide (ITO) as a model substrate on T4 bacteriophage adsorption. A comparative T4 bacteriophage adsorption study shows a significant difference in bacteriophage adsorption observed on chemically modified planar ITO when compared to similarly modified particulate ITO, which infers that trends observed in virus particle interaction studies are not necessarily transferrable to predict virus-planar surface adsorption behaviour. We also found that ITO surfaces modified with methyl groups, (resulting in increased surface roughness and hydrophobicity) remained capable of adsorbing T4 bacteriophage. The adsorption of T4 onto bare, amine and carboxylic functionalised planar ITO suggests the presence of a unique binding behaviour involving specific functional groups on planar ITO surface beyond the non-specific electrostatic interactions that dominate phage to particle interactions. The paper demonstrates the significance of physicochemical properties of surfaces on bacteriophage-surface interactions. (C) 2016 ElsevierInc. All rights reserved.

【 授权许可】

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