Lubrication synergy: Mixture of hyaluronan and dipalmitoylphosphatidylcholine (DPPC) vesicles

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:488

Article

Raj, Akanksha¹  Wang, Min¹  Zander, Thomas²  Wieland, D. C. Florian²  Liu, Xiaoyan^1,5  An, Junxue¹  Garamus, Vasil M.²  Willumeit-Roemer, Regine²  Fielden, Matthew³  Claesson, Per M.^1,4  Dedinaite, Andra^1,4 

[1] KTH Royal Inst Technol, Sch Chem Sci & Engn, Dept Chem Surface & Corros Sci, Drottning Kristinas Vag 51, SE-10044 Stockholm, Sweden

[2] Helmholtz Zentrum Geesthacht, Inst Mat Res, Max Planck Str 1, D-21502 Geesthacht, Germany

[3] KTH Royal Inst Technol, Dept Appl Phys, Albanova Campus,Roslagstullsbacken 21, SE-10044 Stockholm, Sweden

[4] SP Tech Res Inst Sweden, SP Chem Mat & Surfaces, Box 5607, SE-11486 Stockholm, Sweden

[5] Tech Univ Denmark, Dept Chem, Kemitorvet 207, DK-2800 Lyngby, Denmark

关键词: Dipalmitoylphosphatidylcholine (DPPC);    Hyaluronan;    Adsorption;    Self-assembly;    QCM-D;    AFM imaging;    Surface forces;    Friction;    Lubrication;    Small-angle X-ray scattering;   

DOI  :  10.1016/j.jcis.2016.10.091

来源: Elsevier

【 摘 要 】

Phospholipids and hyaluronan have been implied to fulfil important roles in synovial joint lubrication. Since both components are present in synovial fluid, self-assembly structures formed by them should also be present. We demonstrate by small angle X-ray scattering that hyaluronan associates with the outer shell of dipalmitoylphophatidylcholine (DPPC) vesicles in bulk solution. Further, we follow adsorption to silica from mixed hyaluronan/DPPC vesicle solution by Quartz Crystal Microbalance with Dissipation measurements. Atomic Force Microscope imaging visualises the adsorbed layer structure consisting of non-homogeneous phospholipid bilayer with hyaluronan/DPPC aggregates on top. The presence of these aggregates generates a long-range repulsive surface force as two such surfaces are brought together. However, the aggregates are easily deformed, partly rearranged into multilayer structures and partly removed from between the surfaces under high loads. These layers offer very low friction coefficient (<0.01), high load bearing capacity (approximate to 23 MPa), and self-healing ability. Surface bound DPPC/hyaluronan aggregates provide a means for accumulation of lubricating DPPC molecules on sliding surfaces. (C) 2016 The Author(s). Published by Elsevier Inc.

【 授权许可】

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