A functional genomic screen reveals novel host genes that mediate interferon-alpha's effects against hepatitis C virus

JOURNAL OF HEPATOLOGY

卷:56

Article

Zhao, Hong^1,2  Lin, Wenyu¹  Kumthip, Kattareeya¹  Cheng, Du¹  Fusco, Dahlene N.¹  Jilg, Nikolaus¹  Tai, Andrew W.³  Goto, Kaku¹  Zhang, Leiliang¹  Hide, Winston⁴  Jang, Jae Young¹  Peng, Lee F.¹  Chung, Raymond T.¹ 

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Med,Gastrointestinal Unit, Boston, MA 02114 USA

[2] Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China

[3] Univ Michigan Hlth Syst Ann Arbor, Dept Med, Ann Arbor, MI 48105 USA

[4] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

关键词: HCV;    IFN-alpha;    siRNA;    SART1;    U4/U6.U5tri-snRNP;   

DOI  :  10.1016/j.jhep.2011.07.026

来源: Elsevier

【 摘 要 】

Background & Aims: The precise mechanisms by which IFN exerts its antiviral effect against HCV have not yet been elucidated. We sought to identify host genes that mediate the antiviral effect of IFN-alpha by conducting a whole-genome siRNA library screen. Methods: High throughput screening was performed using an HCV genotype 1b replicon, pRep-Feo. Those pools with replicate robust Z scores >= 2.0 entered secondary validation in full-length OR6 replicon cells. Huh7.5.1 cells infected with JFH1 were then used to validate the rescue efficacy of selected genes for HCV replication under IFN-alpha treatment. Results: We identified and confirmed 93 human genes involved in the IFN-alpha anti-HCV effect using a whole-genome siRNA library. Gene ontology analysis revealed that mRNA processing (23 genes, p = 2.756e-22), translation initiation (nine genes, p = 2.42e-6), and IFN signaling (five genes, p = 1.00e-3) were the most enriched functional groups. Nine genes were components of U4/U6.U5 tri-snRNP. We confirmed that silencing squamous cell carcinoma antigen recognized by T cells (SART1), a specific factor of tri-snRNP, abrogates IFN-alpha's suppressive effects against HCV in both replicon cells and JFH1 infectious cells. We further found that SART1 was not IFN-alpha inducible, and its anti-HCV effector in the JFH1 infectious model was through regulation of interferon stimulated genes (ISGs) with or without IFN-alpha. Conclusions: We identified 93 genes that mediate the anti-HCV effect of IFN-alpha through genome-wide siRNA screening; 23 and nine genes were involved in mRNA processing and translation initiation, respectively. These findings reveal an unexpected role for mRNA processing in generation of the antiviral state, and suggest a new avenue for therapeutic development in HCV. (C) 2011 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

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