JOURNAL OF HEPATOLOGY | 卷:42 |
Hepatitis B virus X protein promotes cell migration by inducing matrix metalloproteinase-3 | |
Article | |
Yu, FL ; Liu, HJ ; Lee, JW ; Liao, MH ; Shih, WL | |
关键词: hepatitis B virus; HBV X protein; hepatocellular carcinoma; matrix metalloproteinase-3; cDNA microarray; | |
DOI : 10.1016/j.jhep.2004.11.031 | |
来源: Elsevier | |
【 摘 要 】
Background/Aims: Hepatitis B virus (HBV), a major causative agent of hepatocellular carcinoma (HCC), encodes an oncogenic X protein (HBx) that transcriptionally activates multiple viral and cellular promoters. How this regulation influences HCC is unclear. Methods: Global gene expression in HBx-expressing and non-expressing hepatoma cells was analyzed using cDNA microarrays. Results: Genes that were markedly up- or down-regulated in the presence of HBx included those involved in signal transduction, metabolism, apoptosis, cytokine production, cell cycle, cell adhesion and oncogenesis. Other genes of ill-defined function were also affected. Trans-activation proficient HBx up-regulated the transcription, translation and secretion of matrix metalloproteinase-3 (MMP-3), manifest as a cell migratory phenotype. This HBx effect was abrogated in the presence of a MMP-3 specific peptide inhibitor. Conclusions: HBx exerts selective transcriptional control in hepatoma cells and induces cellular migration through the activation of MMP-3. (C) 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
【 授权许可】
Free
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
10_1016_j_jhep_2004_11_031.pdf | 336KB | download |